CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇 ETS 和 ETS 样基因的特征

• 批准号: 3874727
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874727
• 关键词: Drosophilidae; chickens; chimeric proteins; complementary DNA; early embryonic stage; fusion gene; gene expression; genetic library; genetic mapping; genetic transcription; genetic transduction; microinjections; molecular cloning; mutant; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; oncogenes; protooncogene; transfection; transposon /insertion element; viral carcinogenesis; virus DNA; virus genetics

Three Drosophila genes with cellular sequences related to the ets region of the chicken retrovirus, E26, have been isolated in this laboratory. The characterized portion of the most related gene, D-ets, shows over 90% identity with the last two exons of the chicken c-ets-1 gene at the predicted amino acid level. The region of D-ets (a genomic clone) 5' to this region has been shown to be present in a cDNA clone, and this sequence is divergent from the chicken c-ets-1 gene. D-ets is expressed as a 4.7 kb transcript throughout development. It is localized to chromosome 3R at position 58A/B. D-elg (Drosophila ets-like gene) is a related gene that has been localized to chromosomal position 97D on chromosome 3R. This gene is represented by a clone isolated from a pupae cDNA library. Its sequence shows 60% amino acid similarity with D-ets. D-elg is expressed as a maternal single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. A third gene, E13B, was identified by hybridization with v-ets, and a genomic clone has been partially sequenced. A cDNA clone that hybridized to E13B shows a long Poly A+ tail and is also being sequenced. The E13B gene is expressed as a 1.6 kb RNA species in pupae and adults only. It is located on chromosome 3L at position 66A. Control microinjection studies are beginning that will measure viability and fertility of injected embryos. A cDNA library has been constructed in order to find a full-length D-ets clone. Mapping of D-ets and D-elg by Sl and primer extension analyses are underway.

与果蝇Ets区相关的三个果蝇基因 鸡E26逆转录病毒已在该实验室分离到。这个 最相关的基因D-ETS的特征部分显示90%以上 与鸡c-ETS-1基因最后两个外显子的同源性 预测的氨基酸水平。D-ETS(基因组克隆)5‘端至 这个区域已经被证明存在于一个cdna克隆中，并且这个序列 与鸡c-ETS-1基因不同。D-ETS表示为4.7 kb 在整个发育过程中的文字记录。它被定位在染色体3R的 D-ELG(果蝇ETS样基因)是一个相关基因，具有 定位于染色体3R上的染色体位置97D。这个基因是 由从蚕蛹cdna文库中分离的克隆表示。ITS序列 氨基酸序列与D-ETS有60%的相似性。D-ELG表示为 全程表达的2.0kb的母系单一转录本 胚胎发育，以及蛹和成虫期，但幼虫不存在。一个 第三个基因E13B通过与v-ETS的杂交鉴定，以及一个基因组 克隆已经被部分测序。与E13B杂交的cDNA克隆 显示了一条长长的Poly A+尾巴，也正在进行测序。E13B基因是 仅在蛹和成虫中表达为1.6kb的RNA物种。它位于 位于染色体3L的66A位。对照微量注射研究是 从一开始，这将测量注射胚胎的活力和繁殖力。一个 构建了全长D-ETS的cDNA文库 克隆人。D-ETS和D-ELG的Sl定位和引物延伸分析 正在进行中。