CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

三个果蝇基因的细胞序列相关的ets区域 鸡逆转录病毒E26是在本实验室分离到的。的 最相关的基因D-ets的特征部分显示超过90% 与鸡c-ets-1基因的最后两个外显子的同一性 预测氨基酸水平。D-ets（基因组克隆）5'至 该区域已显示存在于cDNA克隆中，并且该序列 与鸡的c-ets-1基因不同。D-ets表示为4.7 kb 在整个发展过程中。它位于染色体3R， 位置58 A/B。D-elg（Drosophila ets-like gene）是一个与果蝇基因组相关的基因， 定位于染色体3R上的染色体位置97 D。该基因 由分离自puplasticcDNA文库的克隆表示。其序列 与D-ets的氨基酸相似性为60%。D-elg表示为 在整个细胞中表达的2.0 kb的母体单转录本 胚胎发生，以及蛹和成虫阶段，但没有幼虫。一 第三个基因E13 B通过与v-ets杂交鉴定， 克隆已部分测序。与E13 B杂交的cDNA克隆 显示了一条长的Poly A+尾，也正在测序中。E13 B基因是 仅在蛹和成虫中表达为1.6kb RNA种类。它位于 在染色体3L的位置66 A上。对照显微注射研究是 开始测量注射胚胎的生存能力和生育能力。一 为了寻找全长D-ets，构建了cDNA文库 分身通过Sl和引物延伸分析对D-ets和D-elg进行定位。 正在进行中

Three Drosophila genes with cellular sequences related to the ets region of the chicken retrovirus, E26, have been isolated in this laboratory. The characterized portion of the most related gene, D-ets, shows over 90% identity with the last two exons of the chicken c-ets-1 gene at the predicted amino acid level. The region of D-ets (a genomic clone) 5' to this region has been shown to be present in a cDNA clone, and this sequence is divergent from the chicken c-ets-1 gene. D-ets is expressed as a 4.7 kb transcript throughout development. It is localized to chromosome 3R at position 58A/B. D-elg (Drosophila ets-like gene) is a related gene that has been localized to chromosomal position 97D on chromosome 3R. This gene is represented by a clone isolated from a pupae cDNA library. Its sequence shows 60% amino acid similarity with D-ets. D-elg is expressed as a maternal single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. A third gene, E13B, was identified by hybridization with v-ets, and a genomic clone has been partially sequenced. A cDNA clone that hybridized to E13B shows a long Poly A+ tail and is also being sequenced. The E13B gene is expressed as a 1.6 kb RNA species in pupae and adults only. It is located on chromosome 3L at position 66A. Control microinjection studies are beginning that will measure viability and fertility of injected embryos. A cDNA library has been constructed in order to find a full-length D-ets clone. Mapping of D-ets and D-elg by Sl and primer extension analyses are underway.