CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇中与ets相关的三个细胞序列基因 鸡逆转录病毒E26区，已被分离，在此 实验室 最相关的基因D-ets的特征部分， 与鸡c-ets-1基因最后两个外显子的同源性超过90 基因在预测的氨基酸水平。 D-ets区域（基因组 克隆）5 ′的cDNA克隆中已显示存在于该区域， 并且该序列与鸡c-ets-1基因不同。 D-ets是 在整个发育过程中表达为4.7kb转录物。 它是本地化的 位于染色体3R的58 A/B位。 D-elg（Drosophila ets-like gene）是一种 一个相关的基因，已被定位到染色体位置97 D上， 3R染色体。 这个基因是从一个脓包中分离出来的克隆 cDNA文库 其氨基酸序列与D-ets的同源性为60%。 D-elg表达为2.0kb的单个转录本， 在整个胚胎发生，以及蛹和成年阶段，但没有 从幼虫。 D-elg在所有胚胎细胞中表达， 转录激活，没有区域定位。 第三 通过与v-ets的杂交，鉴定了E13 B基因， 克隆已部分测序。 与E13 B杂交的cDNA克隆 显示了一条长的Poly A+尾，也正在测序中。 E13 B基因是 仅在蛹和成虫中表达为1.6kb RNA种类。 是 位于染色体3L的66 A位。

Three Drosophila genes with cellular sequences related to the ets region of the chicken retrovirus, E26, have been isolated in this laboratory. The characterized portion of the most related gene, D-ets, shows over 90% identity with the last two exons of the chicken c-ets-1 gene at the predicted amino acid level. The region of D-ets (a genomic clone) 5' to this region has been shown to be present in a cDNA clone, and this sequence is divergent from the chicken c-ets-1 gene. D-ets is expressed as a 4.7 kb transcript throughout development. It is localized to chromosome 3R at position 58A/B. D-elg (Drosophila ets-like gene) is a related gene that has been localized to chromosomal position 97D on chromosome 3R. This gene is represented by a clone isolated from a pupae cDNA library. Its sequence shows 60% amino acid similarity with D-ets. D-elg is expressed as a single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. D-elg is expressed in all embryonic cells prior to transcriptional activation and has no regional localization. A third gene, E13B, was identified by hybridization with v-ets, and a genomic clone has been partially sequenced. A cDNA clone that hybridized to E13B shows a long Poly A+ tail and is also being sequenced. The E13B gene is expressed as a 1.6 kb RNA species in pupae and adults only. It is located on chromosome 3L at position 66A.