CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇 ETS 和 ETS 样基因的特征

• 批准号: 3853504
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853504
• 关键词: Drosophilidae; RNA splicing; Retroviridae; chickens; developmental genetics; early embryonic stage; gene expression; genetic library; genetic mapping; genetic transcription; molecular cloning; nucleic acid hybridization; nucleic acid sequence; oncogenes; protooncogene; transcription factor; transfection; viral carcinogenesis; virus genetics

Three Drosophila genes with cellular sequences related to the ets region of the chicken retrovirus, E26, have been isolated in this laboratory. The characterized portion of the most related gene, D-ets, shows over 90% identity with the last two exons of the chicken c-ets-1 gene at the predicted amino acid level. The region of D-ets (a genomic clone) 5' to this region has been shown to be present in a cDNA clone, and this sequence is divergent from the chicken c-ets-1 gene. D-ets is expressed as a 4.7 kb transcript throughout development. It is localized to chromosome 3R at position 58A/B. D-elg (Drosophila ets-like gene) is a related gene that has been localized to chromosomal position 97D on chromosome 3R. This gene is represented by a clone isolated from a pupae cDNA library. Its sequence shows 60% amino acid similarity with D-ets. D-elg is expressed as a single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. D-elg is expressed in all embryonic cells prior to transcriptional activation and has no regional localization. A third gene, E13B, was identified by hybridization with v-ets, and a genomic clone has been partially sequenced. A cDNA clone that hybridized to E13B shows a long Poly A+ tail and is also being sequenced. The E13B gene is expressed as a 1.6 kb RNA species in pupae and adults only. It is located on chromosome 3L at position 66A.

三个果蝇基因的细胞序列与ets相关