EFFECTS OF DOXORUBICIN ON CARDIAC DENDRITIC CELLS IN HYPERTENSIVE RATS

用特异性单抗进行组织学和免疫组织化学研究 用抗体来评估慢性阻塞性肺疾病的严重性 心肌病与间质树突状细胞的数量变化 细胞(抗原提呈细胞)，辅助性T淋巴细胞，T- 心脏中的细胞毒/抑制性淋巴细胞和巨噬细胞 阿霉素治疗自发性高血压大鼠(SHR)。在……里面 此外，对答复的修改情况进行了评估 用ICRF-187预处理自发性高血压患者的这些细胞群 预防阿霉素的心脏毒性。通过以下方式获得的结果 细胞计数显示阿霉素治疗后细胞数量显著增加 在间质树突状细胞、辅助性T淋巴细胞和 心脏中的巨噬细胞，这种增加是剂量依赖的，并且 它们会以剂量依赖的方式通过预先处理而减弱 ICRF-187。因此，这项研究表明阿霉素增加了，而不是 减少心脏的某些免疫反应。这些响应将出现 由间质树突状细胞(作为 抗原提呈细胞)，并被认为在 阿霉素心脏毒性的发病机制。

Histologic and immunohistochemical studies using specific monoclonal antibodies were made to evaluate the severity of the chronic cardiomyopathy and the quantitative changes in interstitial dendritic cells (antigen-presenting cells), T-helper lymphocytes, T- cytotoxic/suppressor lymphocytes and macrophages in the hearts of spontaneously hypertensive rats (SHR) treated with doxorubicin. In addition, an assessment was made of the modifications of the responses of these cell populations by pretreatment of the SHR with ICRF-187, which protects against doxorubicin cardiotoxicity. The results obtained by cell counts showed that doxorubicin treatment induces a marked increase in the numbers of interstitial dendritic cells, T-helper lymphocytes and macrophages in the heart, that such increases are dose-dependent, and that they are attenuated in a dose-dependent fashion by pretreatment with ICRF-187. Thus, this work shows that doxorubicin increases, rather than decreases, certain immune responses of the heart. These responses appear to be initiated by the interstitial dendritic cells (which serve as antigen-presenting cells) and are considered to be of importance in the pathogenesis of the cardiotoxicity induced by doxorubicin.