COLLAGEN CRIMPING AND FIXATION PRESSURE IN PORCINE AORTIC VALVES

猪主动脉瓣中的胶原蛋白压接和固定压力

• 批准号: 3858176
• 负责人: V J FERRANS
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858176
• 关键词: aortic valve; atmospheric pressure; chemical structure; collagen; electron microscopy; microscopy; optical polarization; prosthetic heart valve; tissue /organ preservation

The crimping or waviness of collagen in biological tissues can be measured quantitatively in terms of amplitude and periodicity by means of polarized light microscopy and electron microscopy. In porcine aortic valves prepared for use as bioprosthetic cardiac valves, this crimping was shown to be influenced by the conditions of tissue fixation with low concentrations of glutaraldehyde: when fixation was used with no pressure applied, the crimping was similar to that in the native aortic valve; however, low pressure fixation caused marked reduction in the crimping of much of the collagen, and high pressure fixation almost eliminated it completely in the cords and the mid-cusp region. A technique has been developed for making these observations on intact aortic leaflets.

生物组织中胶原蛋白的卷曲或波纹可以是 通过以下方式定量测量振幅和周期性 偏光显微镜和电子显微镜。 在猪主动脉中 准备用作生物假体心脏瓣膜的瓣膜，这种压接 已被证明受到低组织固定条件的影响 戊二醛浓度：无压力固定时 应用后，卷曲与天然主动脉瓣相似； 然而，低压固定导致卷曲明显减少 大部分胶原蛋白，高压固定几乎消除了它 完全位于帘线和中尖区域。 一项技术已被 开发用于在完整的主动脉瓣上进行这些观察。