SOURCES AND EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN THE BRAIN

在小胶质细胞上进行了实验，小胶质细胞是驻留在 培养自大鼠大脑皮层的中枢神经系统。我们 先前已经表明，这些激活的细胞产生超氧化物 自由基，一种活性氧物种(ROS)。ROS还包括 羟基自由基和过氧化氢。静息时的浓度 小胶质细胞内钙离子约为100 nM。当 将钙离子载体A23187添加到组织介质超氧化物中 自由基阴离子从小胶质细胞释放出来。细胞内最大值 这个钙离子载体可以达到的钙大约是300纳米米。我们 已经表明，较大的淀粉样前体的一部分--β~A4肽 蛋白质被聚集成大的、多个大小的片段 存在激活的小胶质细胞和某些其他多肽。

Experiments have been performed on microglia, the resident macrophage in the central nervous system cultured from cerebral cortices of rat. We have previously shown that these activated cells produce the superoxide radical anion, a reactive oxygen species (ROS). ROS include also the hydroxyl radical and hydrogen peroxide. The resting concentration of intracellular calcium in the microglial cell is about 100 nM. When the calcium ionophore, A23187, is added to the tissue media, superoxide radical anions are released from the microglia. The maximum intracellular calcium that can be reached by this calcium ionophore is about 300 nM. We have shown that beta~A4 peptide, a part of the larger amyloid precursor protein, is aggregated into large, multiple~sized fragments in the presence of activated microglia and certain other peptides.