SOURCES AND EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN THE BRAIN

大脑中活性氧中间体的来源和作用

• 批准号: 3782309
• 负责人: D L GILBERT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3782309
• 关键词: calcium channel; free radical oxygen; hydrogen peroxide; hydroxyl radical; intracellular transport; laboratory rat; microglia; superoxides; tissue /cell culture; voltage /patch clamp

Experiments have been performed on microglia, the resident macrophage in the central nervous system cultured from cerebral cortices of rat. We have previously shown that these activated cells produce the superoxide radical anion, a reactive oxygen species (ROS). ROS include also the hydroxyl radical and hydrogen peroxide. The resting concentration of intracellular calcium in the microglial cell is about 100 nM. When the calcium ionophore, A23187, is added to the tissue media, superoxide radical anions are released from the microglia. The maximum intracellular calcium that can be reached by this calcium ionophore is about 300 nM. We have shown that beta~A4 peptide, a part of the larger amyloid precursor protein, is aggregated into large, multiple~sized fragments in the presence of activated microglia and certain other peptides.

实验已经在小胶质细胞上进行，小胶质细胞是脑内的常驻巨噬细胞。 从大鼠大脑皮层培养中枢神经系统。 我们 先前已经证明，这些激活的细胞产生超氧化物， 自由基阴离子、活性氧物质（ROS）。 ROS还包括 羟基自由基和过氧化氢。 静息浓度 小胶质细胞中的细胞内钙约为100 nM。 当 将钙离子载体A23187添加到组织培养基中，超氧化物 自由基阴离子从小胶质细胞释放。 最大细胞内 该钙离子载体可到达的钙为约300 nM。 我们 已经表明β ~A4肽，一种较大的淀粉样蛋白前体的一部分， 蛋白质，聚集成大的，多个大小的片段， 存在活化的小胶质细胞和某些其他肽。