SOURCES AND EFFECTS OF REACTIVE OXYGEN INTERMEDIATES IN THE BRAIN

大脑中活性氧中间体的来源和作用

• 批准号: 3846175
• 负责人: D L GILBERT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3846175
• 关键词: calcium channel; cerebral cortex; cytotoxicity; dopamine; free radical oxygen; glutathione peroxidase; hydrogen peroxide; iron poisoning; laboratory rat; microglia; neurogenesis; potassium; superoxides; tissue /cell culture; voltage /patch clamp; voltage gated channel

Experiments have been performed on microglia, the resident macrophage in the central nervous system (CNS) cultured from rat cerebral cortex. We have previously shown that these activated cells produce the superoxide radical anion, a reactive oxygen species (ROS). ROS also includes the hydroxyl radical and hydrogen peroxide. Earlier, we found that calcium ions can enter microglial cells when these cells are hyperpolarized. We have continued our studies using the patch-clamp technique in cultured microglial cells on the L-type voltage-dependent calcium channels which open when these cells become depolarized. These channels may play a role in our finding that potassium can modulate the production of the superoxide radical anion from microglial cells. We are also continuing our studies with iron toxicity and its relationship to the production of the highly toxic hydroxyl radical. Survival of dopaminergic neurons grown in the presence of glutathione peroxidase show more diffuse neuronal branching, indicating that hydrogen peroxide is involved.

实验已经在小胶质细胞上进行，小胶质细胞是脑内的常驻巨噬细胞。 从大鼠大脑皮层培养的中枢神经系统（CNS）。 我们 先前已经证明，这些激活的细胞产生超氧化物， 自由基阴离子、活性氧物质（ROS）。 ROS还包括 羟基自由基和过氧化氢。 早些时候，我们发现钙 当这些细胞超极化时，离子可以进入小胶质细胞。 我们 继续我们的研究使用膜片钳技术在培养的 L型电压依赖性钙通道上的小胶质细胞， 当这些细胞去极化时就会打开 这些渠道可能发挥作用 在我们的发现中，钾可以调节 来自小胶质细胞的超氧自由基阴离子。 我们还在继续 我们的研究与铁毒性及其关系的生产 剧毒的羟基自由基 多巴胺能神经元的存活 生长在谷胱甘肽过氧化物酶的存在下， 神经元分支，表明过氧化氢参与。