CHARACTERIZATION OF THE WHHL RABBIT LIPOPROTEIN PHENOTYPES

WHHL 兔脂蛋白表型的表征

• 批准号: 3785651
• 负责人: D E BARNARD
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3785651
• 关键词: animal breeding; atherosclerosis; blood lipoprotein; coronary disorder; disease /disorder model; gel electrophoresis; genetic strain; hyperlipidemia; laboratory rabbit; phenotype; veterinary science

The Veterinary Resources Program, National Center for Research Resources, has established a Watanabe hyperlipidemic (WHHL) rabbit breeding colony as a resource for investigators. Watanabe et al reported that there are two types of WHHL rabbits--those with early development and high incidence of coronary atherosclerosis (type 1) and those with late development and low incidence of coronary atherosclerosis (type 2). It has also been shown that either type of rabbit can be selectively bred to produce progeny exhibiting the phenotype of interest. Recently, it was reported that these two types of WHHL rabbits can be differentiated by their lipoprotein phenotypes. Rabbits were fasted overnight and blood was collected in EDTA vacutainers the next morning. Agarose gel electrophoresis was used to identify the lipoprotein phenotypes. Electrophoretic analysis of 14 rabbits from the NCRR WHHL rabbit colony showed that there are two lipoprotein phenotypes: one with an increased density of beta-lipoprotein and another with a beta-doublet. These phenotypes are similar to those reported for the two types of WHHL rabbits. Characterization of the NCRR WHHL rabbits as to phenotype will be of value to investigators who want to use them for their studies.

兽医资源计划，国家研究资源中心， 已经建立了渡边高脂血症(WHHL)兔繁育群体 作为调查人员的资源。渡边等人报告说，有 两种类型的WHHL兔--早期发育和高发育兔 冠状动脉粥样硬化(1型)和晚期冠状动脉粥样硬化症的发生率 发展和低发生率的冠状动脉粥样硬化(2型)。它 也有研究表明，无论哪种类型的兔子都可以有选择地繁殖 产生具有感兴趣的表型的后代。最近，它 据报道，这两种类型的WHHL兔可以区分 根据他们的脂蛋白表型。 兔子禁食过夜，并在EDTA吸尘器中采集血液 第二天早上。琼脂糖凝胶电泳法鉴定 脂蛋白表型。来自中国的14只兔的电泳谱分析 NCRR WHHL兔群体显示有两种脂蛋白表型： 一种是β-脂蛋白密度增加，另一种是 贝塔双打。这些表型与已报道的两种表型相似。 WHHL兔的类型。NCRR WHHL兔TO的特性 表型对于想要利用它们进行研究的研究人员很有价值 他们的研究。