CHARACTERIZATION OF THE WHHL RABBIT LIPOPROTEIN PHENOTYPES

WHHL 兔脂蛋白表型的表征

• 批准号: 2583029
• 负责人: D E BARNARD
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2583029
• 关键词: animal colony; apolipoproteins; atherosclerosis; blood chemistry; blood lipoprotein; coronary disorder; disease /disorder model; familial hyperlipoproteinemia; gel electrophoresis; laboratory rabbit; low density lipoprotein receptor; phenotype

The Veterinary Resources Program, National Center for Research Resources, has established a Watanabe hereditable hyperlipidemic (WHHL) rabbit breeding colony as a resource for investigators. Watanabe et al. reported that there are two types of WHHL rabbits: type 1, those with premature coronary atherosclerosis; and type 2, those with slow development of coronary atherosclerosis. Both types of WHHL rabbits are characterized by high cholesterol and triglyceride concentrations. The two types of WHHL rabbits can be differentiated by their lipoprotein phenotypes. Agarose gel electrophoresis was used to determine the plasma lipoprotein electrophoretic patterns of 14 rabbits from the NCRR WHHL rabbit colony. The results showed that there are at least two lipoprotein phenotypes in the NCRR WHHL rabbit colony, and one is characterized by beta-VLDL. Findings by other investigators showed the properties of beta-VLDL isolated from WHHL rabbits are similar to those observed in humans with type III hyperlipidemia. The data suggest that there is a population of WHHL rabbits with both an apoprotein E and LDL receptor defect.

兽医资源计划，国家研究资源中心， 建立了一个渡边遗传性高脂血症（WHHL）兔 作为研究人员的资源。 Watanabe等人 据报道，有两种类型的WHHL兔：1型，那些与 过早的冠状动脉粥样硬化; 2型，那些缓慢的 冠状动脉粥样硬化的发展。 这两种类型的WHHL兔是 其特征在于高胆固醇和甘油三酯浓度。 的 两种类型的WHHL兔可通过其脂蛋白来区分 表型 琼脂糖凝胶电泳法测定血浆脂蛋白 来自NCRR WHHL兔群的14只兔的电泳图谱。 结果表明，在正常人中至少存在两种脂蛋白表型， NCRR WHHL兔集落，一个以β-VLDL为特征。 其他研究者的发现表明， 从WHHL兔中分离出的抗体类似于在人类中观察到的抗体， III型高脂血症。 数据表明，有一个人口 载脂蛋白E和LDL受体缺陷的WHHL兔