CHARACTERIZATION OF THE WHHL RABBIT LIPOPROTEIN PHENOTYPES

兽医资源计划，国家研究中心 资源，已经建立了渡边遗传性高血压病 （WHHL）兔子繁殖群作为研究人员的资源。 Watanabe等人报告称，WHHL家兔有两种类型： 1型，具有过早冠状动脉粥样硬化的那些;和2型， 冠状动脉粥样硬化进展缓慢者。 两种类型 的WHHL兔以高胆固醇和甘油三酯为特征 浓度的 两种类型的WHHL兔可以区分 通过他们的脂蛋白表型。 采用琼脂糖凝胶电泳法测定血浆 来自NCRR WHHL的14只家兔的脂蛋白电泳图谱 兔子殖民地 结果显示，至少有两个 NCRR WHHL兔群中的脂蛋白表型， 以β-VLDL为特征。 其他研究人员的发现显示， 从WHHL兔中分离的β-VLDL的性质与 在III型高脂血症患者中观察到的那些。 数据 表明有一个WHHL兔群体， 载脂蛋白E和LDL受体缺陷。 从WHHL兔VLDL中分离载脂蛋白E，以确定 这是次品。 NCRR WHHL载脂蛋白E的鉴定 兔可导致III型高脂血症的动物模型。 鉴别这两种类型的WHHL兔将是非常宝贵的， 科学界，因为它将消除一个 当WHHL家兔作为实验室时，可能的混杂变量 动物模型

The Veterinary Resources Program, National Center for Research Resources, has established a Watanabe hereditable hyperlipidemic (WHHL) rabbit breeding colony as a resource for investigators. Watanabe et al. reported that there are two types of WHHL rabbits: type 1, those with premature coronary atherosclerosis; and type 2, those with slow development of coronary atherosclerosis. Both types of WHHL rabbits are characterized by high cholesterol and triglyceride concentrations. The two types of WHHL rabbits can be differentiated by their lipoprotein phenotypes. Agarose gel electrophoresis was used to determine the plasma lipoprotein electrophoretic patterns of 14 rabbits from the NCRR WHHL rabbit colony. The results showed that there are at least two lipoprotein phenotypes in the NCRR WHHL rabbit colony, and one is characterized by beta-VLDL. Findings by other investigators showed the properties of beta-VLDL isolated from WHHL rabbits are similar to those observed in humans with type III hyperlipidemia. The data suggest that there is a population of WHHL rabbits with both an apoprotein E and LDL receptor defect. Apoprotein E is being isolated from WHHL rabbit VLDL to determine if it is defective. Characterization of apoprotein E from NCRR WHHL rabbits may result in an animal model for type III hyperlipidemia. Identification of the two types of WHHL rabbits will be invaluable to the scientific community because it will eliminate the source of a possible confounding variable when WHHL rabbits are the laboratory animal model.