ANEUPLOIDY AND CELL CYCLE FRACTIONS IN BENIGN AND MALIGNANT TUMORS

良性和恶性肿瘤中的非整倍性和细胞周期分数

• 批准号: 3796581
• 负责人: M STETLER-STEVENSON
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3796581
• 关键词: DNA; aneuploidy; bladder neoplasm; cellular oncology; cellular pathology; diploidy; dyes; human tissue; interstitial cystitis; method development; monoclonal antibody; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; prognosis; stainings; tissue /cell preparation; transitional cell carcinoma; urinary bladder epithelium

We have studied the presence of aneuploidy, S phase and hyperdiploid fractions in bladder washings from patients with transitional carcinoma and benign cystitis. We have found apparent false aneuploid populations in bladder washings from patients with benign cystitis. We have investigated the source of this apparent aneuploid population. We have studied DNA dye saturation kinetics and examined the "NIM" or nuclear isolation method vs. the Vindelov method and have found the Vindelov to be superior. We are currently using monoclonal antibodies to focus on the urothelium and to eliminate from our analysis the reactive cells which may have different nuclear characteristics that lead to differential staining and false aneuploidy. Also, fixatives are being examined to determine the best method to preserve cells for transport to a reference laboratory or until analysis can be performed. Ethanol appears to provide superior coefficients of variation (C.V. indicates quality of analysis and sensitivity of detection of aneuploid populations) compared to methanol and paraformaldehyde. We are still investigating modifications of our ethanol fixation method to improve coefficients of variation. We are also studying the presence of aneuploidy, S phase and hyperdiploid fractions in gastrinoma tissues from 60 patients and correlating these results with clinical prognosis. We developed a method for DNA content analysis from paraffin embedded tissues for this purpose.

我们研究了非整倍体、S期和超二倍体的存在 移行细胞癌患者膀胱冲洗液中的成分， 良性膀胱炎 我们发现了明显的假非整倍体群体， 良性膀胱炎患者的膀胱冲洗液。 我们已经调查 这个明显的非整倍体群体的来源。 我们研究了DNA染料 饱和动力学，并检查了“NIM”或核分离方法与 Vindelov方法，并发现Vindelov方法更上级。 我们 目前使用单克隆抗体来集中于尿道炎， 从我们的分析中排除可能具有不同 导致差异染色和假染色的核特征 非整倍性 此外，固定剂正在审查，以确定最佳的 保存细胞以运输至参考实验室或直至 可以进行分析。 乙醇似乎提供了上级 变异系数（C.V.表示分析质量， 检测非整倍体群体的灵敏度）与甲醇相比， 多聚甲醛 我们仍在研究乙醇的改良 固定法提高变异系数。 我们也在研究 非整倍体、S期和超二倍体部分的存在， 胃泌素瘤组织，并将这些结果与 临床预后 我们开发了一种DNA含量分析方法， 石蜡包埋组织用于此目的。