EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS

淋巴增殖性疾病中癌基因的表达

• 批准号: 3808581
• 负责人: M STETLER-STEVENSON
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3808581
• 关键词: T lymphocyte; chromosome aberrations; chromosome translocation; gene expression; genetic manipulation; genetic regulation; genetic transcription; human genetic material tag; human tissue; lymphoma; natural gene amplification; neoplasm /cancer genetics; northern blottings; oncogenes; polymerase chain reaction; tissue /cell culture

We have continued our studies of the expression of bcl-2 in follicular lymphoma. We developed a procedure for isolation of RNA followed by reverse transcription and PCR amplification of bcl-2/heavy chain sequences. Using this method, we are able to measure the level of expression of bcl-2 in small specimens. We have compared our technique in cell lines carrying the t(14;18) with the standard procedure of Northern blot analysis and have documented the validity of our method. Using this procedure, we have measured the levels of expression of bcl-2 in a series of biopsy specimens. We are currently correlating our results with clinical outcome. Studies indicate that bcl-2 may incur a growth advantage to cells but that involvement of other oncogenes, especially myc, is necessary to attain full malignant potential. We have developed a similar technique utilizing PCR to measure myc transcripts and have evaluated the validity of this method using non-stimulated and PHA stimulated peripheral blood T cells and comparing our PCR results to Northern blot data. We are studying the expression of myc in the follicular lymphomas studied for bcl-2 "expression. In addition, we are studying the expression of myc in lymphomas that have progressed to a more aggressive phenotype to determine if myc expression is associated with this phenotype. This will provide important information as to the interrelationship of various oncogenes in the development of follicular lymphoma.

我们继续对bcl-2在卵泡中的表达进行研究