FLOW CYTOMETRIC ANALYSIS OF BENIGN AND MALIGNANT TUMORS
良性和恶性肿瘤的流式细胞术分析
基本信息
- 批准号:5201032
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:B lymphocyte aneuploidy breast neoplasm /cancer diagnosis breast neoplasms cell cycle cellular oncology cellular pathology computer simulation flow cytometry gene expression human genetic material tag human tissue lymphoma method development neoplasm /cancer diagnosis neoplasm /cancer genetics prognosis protooncogene tissue /cell culture tumor suppressor genes
项目摘要
In lymph node negative breast cancer, a strong association has been
shown between S-phase and prognosis. In addition, demonstration of an
aneuploid population has diagnostic implications. We studied the
effect of doublet discrimination on S-phase fraction estimates and
detection of tetraploid populations in fifty breast carcinomas,
peripheral blood lymphocytes and cell lines. Doublet estimates
obtained using computer modeling as well as electronic detection
techniques based upon pulse shape analysis of fluorescent height vs
area or fluorescent width vs area were compared. In biologically
homogeneous (eg cell lines) specimens, all techniques yielded good
results. However, in biologically complex specimens doublet estimates
varied greatly (up to five fold difference) depending upon the method
used. This variation in doublet subtraction affected estimates of
proliferating cells.
Light chain restriction (detection of monoclonal B-cells) is
synonymous with B-cell neoplasia in most clinical situations. We
therefore studied flow cytometric methods for light chain detection in
375 patients and 181 normal controls. We detected monoclonality in 92
of the 375 patient specimens and in no normal controls (results
correlated with clinical outcome). 79 out of 375 (23%) specimens were
grossly monoclonal and detected by standard methods. Of these 79
cases, different sets of anti-light chain reagents had different
sensitivities with one detecting all monoclonal populations but
sensitivities of 66% and 78% obtained with two other sets. In 24
cases negative for monoclonality by standard methods but in which the
patient had a previous history of lymphoma, a clonal search was
performed based upon antigen density and cell size. Monoclonality was
detected in 13 of these 24 cases (54%) demonstrating the superior
sensitivity of this method.
We have established flow cytometric methods to quantitate expression
of c-myc, bcl-2 and p53 protein expression. We will compare these
methods to RT-PCR methods on sorted and unsorted patient specimens.
在淋巴结阴性乳腺癌中,有很强的相关性
显示S期和预后之间。 此外,还演示了一个
非整倍体群体具有诊断意义。 我们研究了
双峰辨别对 S 相分数估计的影响和
检测五十个乳腺癌中的四倍体群体,
外周血淋巴细胞和细胞系。 双峰估计
使用计算机建模和电子检测获得
基于荧光高度与脉冲形状分析的技术
面积或荧光宽度与面积进行比较。 在生物学上
均质(例如细胞系)样本,所有技术均产生良好的结果
结果。 然而,在生物复杂的样本中,双峰估计
取决于方法,差异很大(最多五倍差异)
用过的。 双峰减法的这种变化影响了估计
增殖细胞。
轻链限制(检测单克隆 B 细胞)是
在大多数临床情况下与 B 细胞肿瘤同义。 我们
因此研究了流式细胞仪检测轻链的方法
375 名患者和 181 名正常对照。 我们在 92 例中检测到单克隆性
375 例患者标本中没有正常对照(结果
与临床结果相关)。 375 个样本中的 79 个 (23%)
完全单克隆并通过标准方法检测。 其中 79
情况下,不同组的抗轻链试剂有不同的
检测所有单克隆群体的敏感性,但
另外两组的灵敏度分别为 66% 和 78%。 24年
标准方法显示单克隆性呈阴性的情况,但其中
患者既往有淋巴瘤病史,进行克隆搜索
根据抗原密度和细胞大小进行。 单克隆性是
在这 24 例病例中的 13 例 (54%) 中检测到,证明了优越性
该方法的灵敏度。
我们已经建立了流式细胞术方法来定量表达
c-myc、bcl-2 和 p53 蛋白表达。 我们将比较这些
对分类和未分类的患者标本进行 RT-PCR 方法的方法。
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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M STETLER-STEVENSON其他文献
M STETLER-STEVENSON的其他文献
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{{ truncateString('M STETLER-STEVENSON', 18)}}的其他基金
TIMP EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
正常淋巴细胞和淋巴肿瘤中的 TIMP 表达
- 批准号:
5201033 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
淋巴增殖性疾病中癌基因的表达
- 批准号:
3813436 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
淋巴增殖性疾病中癌基因的表达
- 批准号:
3808581 - 财政年份:
- 资助金额:
-- - 项目类别:
ANEUPLOIDY AND CELL CYCLE FRACTIONS IN BENIGN AND MALIGNANT TUMORS
良性和恶性肿瘤中的非整倍性和细胞周期分数
- 批准号:
3796581 - 财政年份:
- 资助金额:
-- - 项目类别:
ANEUPLOIDY AND CELL CYCLE FRACTIONS IN BENIGN AND MALIGNANT TUMORS
良性和恶性肿瘤中的非整倍性和细胞周期分数
- 批准号:
3774419 - 财政年份:
- 资助金额:
-- - 项目类别:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
淋巴增殖性疾病中癌基因的表达
- 批准号:
3796529 - 财政年份:
- 资助金额:
-- - 项目类别:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
正常淋巴细胞和淋巴肿瘤中的 TIMP-1 表达
- 批准号:
3796582 - 财政年份:
- 资助金额:
-- - 项目类别:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
正常淋巴细胞和淋巴肿瘤中的 TIMP-1 表达
- 批准号:
3774420 - 财政年份:
- 资助金额:
-- - 项目类别:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
正常淋巴细胞和淋巴肿瘤中的 TIMP-1 表达
- 批准号:
3752120 - 财政年份:
- 资助金额:
-- - 项目类别:
FLOW CYTOMETRIC ANALYSIS OF BENIGN AND MALIGNANT TUMORS
良性和恶性肿瘤的流式细胞术分析
- 批准号:
3752119 - 财政年份:
- 资助金额:
-- - 项目类别:
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