FLOW CYTOMETRIC ANALYSIS OF BENIGN AND MALIGNANT TUMORS
FLOW CYTOMETRIC ANALYSIS OF BENIGN AND MALIGNANT TUMORS
批准号:
5201032
负责人:
M STETLER-STEVENSON
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
B lymphocyte aneuploidy breast neoplasm /cancer diagnosis breast neoplasms cell cycle cellular oncology cellular pathology computer simulation flow cytometry gene expression human genetic material tag human tissue lymphoma method development neoplasm /cancer diagnosis neoplasm /cancer genetics prognosis protooncogene tissue /cell culture tumor suppressor genes
中文摘要
在淋巴结阴性乳腺癌中,有很强的相关性
英文摘要
In lymph node negative breast cancer, a strong association has been
shown between S-phase and prognosis. In addition, demonstration of an
aneuploid population has diagnostic implications. We studied the
effect of doublet discrimination on S-phase fraction estimates and
detection of tetraploid populations in fifty breast carcinomas,
peripheral blood lymphocytes and cell lines. Doublet estimates
obtained using computer modeling as well as electronic detection
techniques based upon pulse shape analysis of fluorescent height vs
area or fluorescent width vs area were compared. In biologically
homogeneous (eg cell lines) specimens, all techniques yielded good
results. However, in biologically complex specimens doublet estimates
varied greatly (up to five fold difference) depending upon the method
used. This variation in doublet subtraction affected estimates of
proliferating cells.
Light chain restriction (detection of monoclonal B-cells) is
synonymous with B-cell neoplasia in most clinical situations. We
therefore studied flow cytometric methods for light chain detection in
375 patients and 181 normal controls. We detected monoclonality in 92
of the 375 patient specimens and in no normal controls (results
correlated with clinical outcome). 79 out of 375 (23%) specimens were
grossly monoclonal and detected by standard methods. Of these 79
cases, different sets of anti-light chain reagents had different
sensitivities with one detecting all monoclonal populations but
sensitivities of 66% and 78% obtained with two other sets. In 24
cases negative for monoclonality by standard methods but in which the
patient had a previous history of lymphoma, a clonal search was
performed based upon antigen density and cell size. Monoclonality was
detected in 13 of these 24 cases (54%) demonstrating the superior
sensitivity of this method.
We have established flow cytometric methods to quantitate expression
of c-myc, bcl-2 and p53 protein expression. We will compare these
methods to RT-PCR methods on sorted and unsorted patient specimens.
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会议论文
TIMP EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
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批准号:5201033
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
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批准号:3813436
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
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批准号:3808581
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
ANEUPLOIDY AND CELL CYCLE FRACTIONS IN BENIGN AND MALIGNANT TUMORS
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批准号:3796581
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
ANEUPLOIDY AND CELL CYCLE FRACTIONS IN BENIGN AND MALIGNANT TUMORS
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批准号:3774419
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
EXPRESSION OF ONCOGENES IN LYMPHOPROLIFERATIVE DISORDERS
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批准号:3796529
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
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批准号:3796582
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
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批准号:3774420
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
TIMP-1 EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS
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批准号:3752120
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
FLOW CYTOMETRIC ANALYSIS OF BENIGN AND MALIGNANT TUMORS
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批准号:3752119
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:M STETLER-STEVENSON
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依托单位:
海外基金