TIMP EXPRESSION BY NORMAL LYMPHOCYTES AND IN LYMPHOID NEOPLASMS

正常淋巴细胞和淋巴肿瘤中的 TIMP 表达

• 批准号: 5201033
• 负责人: M STETLER-STEVENSON
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201033
• 关键词: B lymphocyte; Burkitt's lymphoma; Retroviridae; apoptosis; athymic mouse; cold temperature; gene expression; human tissue; neoplasm /cancer invasiveness; neoplastic growth; physiologic stressor; tissue /cell culture; tissue inhibitor of metalloproteinases; transfection

We have studied the expression of TIMP-1 in lymphoid tissue. TIMP-1 is not expressed by isolated peripheral blood B-cells or tonsillar B- cells, even after stimulation with a panel of cytokines. TIMP-1 is also not expressed by low grade B-cell lymphoma cell lines. TIMP-1 is, however, expressed by four out of eight Burkitt cell lines studied. In vitro studies indicate that the cell lines expressing TIMP-1 have a higher invasion potential. In the nude mouse, TIMP-1 expressing cell lines are more tumorigenic and invasive. The TIMP-1 negative cell lines are not invasive and have a low incidence of tumor formation. The TIMP-1 expressing cell lines are resistant to cold shock induced apoptosis while the TIMP-1 negative cell lines readily undergo apoptosis. We have infected TIMP-1 negative cell lines with a retroviral construct containing TIMP-1 and have isolated TIMP-1 expressing clones. TIMP-1 expression appears to have increased the growth rate compared to retrovirus alone. We are currently studying the effect of TIMP-1 on invasiveness and apoptosis.

我们研究了TIMP-1在淋巴组织中的表达。TIMP-1 分离的外周血B细胞或扁桃体B细胞不表达 细胞，即使在一组细胞因子的刺激下也是如此。TIMP-1是 在低度恶性B细胞淋巴瘤细胞系中也不表达。TIMP-1 然而，每八个Burkitt细胞系中就有四个表达 学习。体外研究表明，表达基因的细胞系 TIMP-1具有较高的侵袭潜能。在裸鼠体内，TIMP-1 表达细胞系具有更强的致瘤性和侵袭性。TIMP-1 阴性细胞系无侵袭性，肿瘤发生率低 队形。表达TIMP-1的细胞株具有抗寒性 休克诱导细胞凋亡而TIMP-1阴性的细胞系 经历细胞凋亡。我们已经用TIMP-1阴性细胞株感染了 含TIMP-1的逆转录病毒载体及其分离的TIMP-1 表达克隆。TIMP-1的表达似乎增加了 与单纯逆转录病毒相比，生长速度更快。我们目前正在研究 TIMP-1在侵袭性和细胞凋亡中的作用