INFLUENCE OF AGE ON 7-DEHYDROCHOLESTEROL IN THE SKIN

年龄对皮肤中 7-脱氢胆固醇的影响

• 批准号: 3480123
• 负责人: MICHAEL F. HOLICK
• 金额: $ 18.11万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3480123
• 关键词: calcium metabolism; cholecalciferol; fibroblasts; human age group; human subject; keratinization; keratinocyte; light adverse effect; nutrition related tag; skin; solar radiation; sunscreens; ultraviolet radiation; vitamin biosynthesis

The goals of our research program are to define how aging affects the capacity of human skin to provide adequate vitamin D nutrition and to determine whether aging affects the responsiveness of the skin to the active form of vitamin D, 1,25-dihydroxyvitamin D. We have developed the technology and the expertise to (a) synthesis radiolabeled 7-dehydrocholesterol, (b) chromatographically separate 7-dehydrocholesterol from its photoproducts and cholesterol, (c) culture human keratinocytes, and (d) conduct human studies to quantitatively determine the effect of exposure to ultraviolet radiation on the vitamin D endocrine system. During the next 5 years of this grant we plan, in a methodical fashion, to (a) determine the mechanism(s) responsible for the age-dependent decrease in epidermal 7-dehydrocholesterol stores, (b) evaluate whether aging affects the responsiveness of human keratinocytes to respond to 1,25-(OH)2-D3, (c) examine the effect that aging has on the ability of human volunteers to raise circulating concentrations of vitamin D in response to exposure to ultraviolet radiation, (d) determine how much surface area of the body needs to be exposed to solar radiation in order to provide adequate vitamin D nutrition, (e) determine whether a single long exposure or multiple short exposures to UV radiation has the same effect on the vitamin D endocrine system, and (f) determine what effect changes in latitude has on the capacity of the skin to produce vitamin D3. Results from this research should provide basic new insights about how aging affects the capacity of human skin to produce vitamin D3 and to provide valuable new information about how the elderly can use the natural resource of the sun to provide them with adequate vitamin D nutrition when dietary sources are inadequate.

我们研究计划的目标是定义衰老如何影响 人体皮肤提供充足的维生素D营养的能力 确定老化是否会影响皮肤对 维生素D的活性形式，1，25-二羟基维生素D。我们开发了 (A)合成放射性标记的技术和专业知识 7-脱氢胆固醇，(B)色谱分离7-脱氢胆固醇 (C)培养人角质形成细胞， 和(D)进行人体研究，以定量确定 暴露于紫外线辐射对维生素D内分泌系统的影响。 在这笔赠款的未来5年里，我们计划有条不紊地 (一)确定年龄相关性下降的机制(S) 在表皮7-脱氢胆固醇储存中，(B)评估衰老 影响角质形成细胞的反应性 1，25-(OH)2-D3，(C)研究老化对 人类志愿者将提高体内循环中维生素D的浓度 对紫外线照射的反应，(D)确定多少 身体的表面积需要暴露在太阳辐射下，以便 提供充足的维生素D营养，(E)确定是否有单一的长 暴露在紫外线辐射下或多次短期暴露在紫外线辐射下对 维生素D内分泌系统，和(F)决定在 纬度对皮肤产生维生素D3的能力有影响。结果 从这项研究应该提供关于衰老的基本新见解 影响人类皮肤产生维生素D3和提供 关于老年人如何利用自然资源的有价值的新信息 在饮食时为他们提供充足的维生素D营养 消息来源不足。