BIOLOGY OF HUMAN AIDS RETROVIRUS

人类艾滋病逆转录病毒的生物学

• 批准号: 3818247
• 负责人: B CHESEBRO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818247
• 关键词: AIDS; CD4 molecule; antibody neutralization test; antiviral antibody; communicable disease diagnosis; diagnosis design /evaluation; human immunodeficiency virus 1; human subject; immunofluorescence technique; immunologic assay /test; immunoperoxidase; monoclonal antibody; tissue /cell culture; transfection; virus classification; virus envelope; virus protein; virus receptors

The goal of this work has been to develop a sensitive quantitative in vitro focal immunoassay for human immunodeficiency virus (HIV) and HIV-infected cells. This has been approached by inserting the human CD4 gene into human cell lines. Clones with high CD4 expression were then used as targets for HIV infection which was detected by HIV-specific antibodies with indirect immunofluorescence or immunoperoxidase methods. The assay was shown to be HIV-specific by using monoclonal antibodies specific for HIV envelope or core proteins; however, polyclonal human anti- HIV serum was also effective. The assay was highly sensitive, capable of detecting most wild-type HIV strains isolated from patients. Future experiments are aimed at using the assay to study HIV expression in cells of AIDS patients.

这项工作的目标是开发一种敏感的定量方法， 人类免疫缺陷病毒（HIV）体外局灶免疫测定 和艾滋病毒感染的细胞。 这是通过插入 将人CD 4基因导入人细胞系。 高CD 4克隆 然后将表达用作HIV感染的靶标， 通过艾滋病毒特异性抗体检测， 免疫荧光或免疫过氧化物酶方法。 该测定 通过使用单克隆抗体特异性 HIV包膜或核心蛋白;然而，多克隆人抗- HIV血清也有效。 该测定是高度灵敏的， 能够检测大多数野生型HIV毒株， 患者 未来的实验旨在使用该检测方法研究艾滋病毒 在艾滋病患者的细胞中表达。