BIOLOGY OF HUMAN AIDS RETROVIRUS

这个项目的主要目标是确定变化的作用 在HIV序列中， 艾滋病聚合酶基因的变异与耐药相关 某些药物组合已经通过分子克隆进行了研究， 体外耐药性分析。单个氨基酸的作用 这些研究确定了这些变化。变化 已经在体内和体外对包膜基因进行了研究。体内env 对痴呆相关病毒的序列分析表明， 独特的病毒株可能是导致艾滋病毒发生的原因 痴呆相反，其他分析表明，痴呆症不是 与高水平的病毒DNA有关V3的影响 包膜基因区对巨噬细胞嗜性和NSI和SI的影响 表型被映射到单个V3氨基酸，并且发现 通常巨噬细胞嗜性和SI表型是独立的， 由不同V3位置的氨基酸决定。这一发现 提供了一种解释，为艾滋病毒株的发生， SI表型和巨噬细胞嗜性。

The primary goal of this project is to determine the role of variation in HIV sequences in causing the various clinical manifestations seen in AIDS. Variation in the polymerase gene associated with resistance to certain drug combinations have been studied by molecular cloning and in vitro analysis of drug resistance. The role of individual amino acid alterations has been determined by these studies. Variation in the envelope gene has been studied both in vivo and in vitro. In vivo env sequence analysis of viruses associated with dementia suggested that unique viral strains may be responsible for the occurrence of HIV dementia. In contrast, other analyses indicated that dementia was not associated with higher levels of viral DNA. The influence of the V3 region of the envelope gene on macrophage tropism and the NSI and SI phenotypes was mapped to individual V3 amino acids, and it was found that usually macrophage tropism and the SI phenotype were independently determined by amino acids at different V3 positions. This finding provides an explanation for the occurrence of HIV strains which exhibit both the SI phenotype and macrophage tropism.