BIOLOGY OF HUMAN AIDS RETROVIRUS

这个项目的主要目标是确定变量的作用 在HIV序列中引起不同的临床表现 艾滋病。与抗病相关的聚合酶基因变异 某些药物组合已经通过分子克隆和生物信息学研究。 体外耐药分析。单个氨基酸的作用 这些研究已经确定了这些变化。中的变化 包膜基因已经在体内和体外进行了研究。在体环境中 对痴呆症相关病毒的序列分析表明 独特的病毒株可能是艾滋病毒发生的原因 痴呆症。相比之下，其他分析表明，痴呆症并不是 与较高水平的病毒DNA有关。V3的影响 巨噬细胞趋向性的包膜基因区域与NSI和SI 表型被映射到单独的V3氨基酸，并发现 通常巨噬细胞的嗜性和SI表型是独立的 由不同V3位的氨基酸决定。这一发现 提供了出现艾滋病毒毒株的解释，这些毒株表现出 SI表型和巨噬细胞嗜性。

The primary goal of this project is to determine the role of variation in HIV sequences in causing the various clinical manifestations seen in AIDS. Variation in the polymerase gene associated with resistance to certain drug combinations have been studied by molecular cloning and in vitro analysis of drug resistance. The role of individual amino acid alterations has been determined by these studies. Variation in the envelope gene has been studied both in vivo and in vitro. In vivo env sequence analysis of viruses associated with dementia suggested that unique viral strains may be responsible for the occurrence of HIV dementia. In contrast, other analyses indicated that dementia was not associated with higher levels of viral DNA. The influence of the V3 region of the envelope gene on macrophage tropism and the NSI and SI phenotypes was mapped to individual V3 amino acids, and it was found that usually macrophage tropism and the SI phenotype were independently determined by amino acids at different V3 positions. This finding provides an explanation for the occurrence of HIV strains which exhibit both the SI phenotype and macrophage tropism.