IMMUNOBIOLOGY OF EQUINE INFECTIOUS ANEMIA VIRUS, A RETROVIRUS MODEL FOR AIDS

本项目的目标是研究马的抗原变异。 传染性贫血病毒(EIAV)可能的致病机制 通过避免特定的免疫反应而坚持下去。焦点 免疫荧光分析已被用于扩增和生物克隆一个 马、猫和狗细胞系中病毒分离物的数量。在……里面 此外，还分离出了连续发热期的病毒分离株。 从受感染的马血中分离出来的，已经被适应在马身上复制 皮肤细胞系，并已在该细胞系中进行了生物克隆。一 在这些野外分离株中，MA-1正被用来感染一匹马，以便 在体内产生抗原性变体。 正在开发的单抗是为了在抗原性上 确定各种病毒分离株的特征。总共有34个克隆 发现与EIAV感染的细胞通过膜和/或细胞质发生反应 荧光。对这些克隆的进一步定性正在进行中。病毒 Hirt的限制性内切酶分析也对分离株进行了比较。 感染细胞的上清部分。

The goal of this project is to study antigenic variation of equine infectious anemia virus (EIAV) with regard to possible mechanisms of viral persistence through avoidance of the specific immune response. A focal immunofluorescence assay has been used to expand and biologically clone a number of viral isolates in equine, feline and canine cell lines. In addition, viral isolates from sequential febrile periods have been isolated from infected horse blood, have been adapted to replicate in an equine dermal cell line and have been biologically cloned in that cell line. One of these field isolates, MA-1, is being used to infect a horse in order to generate antigenic variants in vivo. Monoclonal antibodies are being developed in order to antigenically characterize the various viral isolates. A total of 34 clones has been found reactive with EIAV-infected cells by membrane and/or cytoplasmic fluorescence. Further characterization of these clones is ongoing. Virus isolates are also being compared by restriction enzyme analyses of Hirt supernatant fractions of infected cells.