IMMUNOBIOLOGY OF EQUINE INFECTIOUS ANEMIA VIRUS, A RETROVIRUS MODEL FOR AIDS

马传染性贫血病毒（艾滋病逆转录病毒模型）的免疫生物学

• 批准号: 4688533
• 负责人: B CHESEBRO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688533
• 关键词: AIDS; Retroviridae; blood cells; disease /disorder model; equine infectious anemia virus; genetic recombination; hemolytic anemia; immunosuppression; monoclonal antibody; virus antigen; virus diseases; virus genetics; virus infection mechanism

The goal of this project is to study antigenic variation of equine infectious anemia virus (EIAV) with regard to possible mechanisms of viral persistence through avoidance of the specific immune response. A focal immunofluorescence assay has been used to expand and biologically clone a number of viral isolates in equine, feline and canine cell lines. In addition, viral isolates from sequential febrile periods have been isolated from infected horse blood, have been adapted to replicate in an equine dermal cell line and have been biologically cloned in that cell line. One of these field isolates, MA-1, is being used to infect a horse in order to generate antigenic variants in vivo. Monoclonal antibodies are being developed in order to antigenically characterize the various viral isolates. A total of 34 clones has been found reactive with EIAV-infected cells by membrane and/or cytoplasmic fluorescence. Further characterization of these clones is ongoing. Virus isolates are also being compared by restriction enzyme analyses of Hirt supernatant fractions of infected cells.

本项目的目的是研究马的抗原变异 传染性贫血病毒（EIAV）的可能机制 通过避免特异性免疫反应而持续存在。 震源 免疫荧光测定已被用于扩增和生物克隆a 马、猫和犬细胞系中病毒分离株的数量。 在 此外，已分离出连续发热期的病毒分离株， 从受感染的马血中分离出来， 真皮细胞系，并已在该细胞系中进行了生物克隆。 一 在这些田间分离株中，MA-1被用来感染马， 在体内产生抗原变体。 正在开发单克隆抗体，以便在抗原性上 鉴定各种病毒分离株。 总共有34个克隆体 发现通过膜和/或细胞质与EIAV感染的细胞反应 荧光。 这些克隆的进一步特征鉴定正在进行中。 病毒 还通过限制性内切酶分析比较了 感染细胞的上清液部分。