DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS

研究生化反应的生物物理方法的发展

• 批准号: 3843292
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3843292
• 关键词: acid base balance; bioengineering /biomedical engineering; biomedical equipment development; blood /plasma substitute; carbon; carbon dioxide; carbon monoxide; chemical binding; chemical reaction; chlorine; hemoglobin; human tissue; infrared spectrometry; microcalorimetry; nuclear magnetic resonance spectroscopy; phosphates; stable isotope; temperature

Work on the development of instrumentation for the evaluation of various hemoglobin derivatives proposed as a blood substitute has proceeded at an accelerated rate with the coming on line of the pilot plant at LAIR which can furnish large amounts of the protein. The oxygen equilibrium curve apparatus is now being tested on human hemoglobin samples and calorimetric measurements of the binding of CO,CO2, and chloride to HbAo and the DBBF- HB product are underway. The pulsed accelerated flow apparatus is nearly ready for operation. Of considerable importance in evaluating the product's suitability for injection into humans is the purity of the material, a precise knowledge of the oxygen equilibrium curve and the protein's response to its various effectors, i.e. chloride, pH, temperature, phosphate, CO2, as well as oxygen concentration. Binding isotherms of oxygen and carbon monoxide in the presence and absence of these effectors are being determined both manometrically and optically as well as in the microcalorimeter. CO2 binding curves are being determined calorimetrically and by C13 NMR. The physical chemistry of hemoglobin has suffered in the past from the lack of a suitable standard state from which to start the free energy calculations. These studies on the isoionic protein, i.e. in the absence of all effectors, will allow such a standard state to be established.

开发用于评估各种生物多样性的仪器的工作 作为血液替代品的血红蛋白衍生物的研究进展 随着LAIR中试工厂的上线， 可以提供大量的蛋白质。 氧平衡曲线 该仪器目前正在对人类血红蛋白样本进行测试， 测量CO、CO2和氯化物与HbAo和DBBF的结合。 HB产品正在开发中。 脉冲加速流装置几乎是 准备运行。 在评估中具有相当重要的意义， 产品是否适合注射到人体内，取决于产品的纯度。 材料，氧平衡曲线的精确知识和 蛋白质对其各种效应物的反应，即氯化物，pH， 温度、磷酸盐、CO2以及氧气浓度。 结合 氧和一氧化碳在存在和不存在的等温线 这些效应器通过测压和光学测定， 和微量热计一样。 CO2结合曲线正在确定中 通过量热法和C13 NMR测定。 血红蛋白的物理化学性质 在过去，由于缺乏一个合适的标准状态， 开始自由能计算。 这些关于等离子体的研究 蛋白质，即不存在所有效应物，将允许这样的标准 国家要建立。