DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES

开发用于溶液和细胞生化研究的生物量热计

• 批准号: 3878934
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3878934
• 关键词: NAD nucleosidase; microcalorimetry; phospholipids; reagent /indicator; thermodynamics

Studies of the reaction of NAD-NADASE have been extended on the all tantalum stopped-flow microcalorimeter to 10 degrees C, 25 degrees C, and 37 degrees C confirming the endothermic nature of the reaction by demonstrating the 5-fold increase in activity from 25 degrees C to 10 degrees C with the almost complete loss in activity at 37 degrees C. A new heat capacity measuring microcalorimeter has been developed which is capable of measuring charges of 1 part in one hundred thousand of total heat capacity of phospholipids. A reaction heat of 60 calories/mole was found as they went from multilamellar vesicles to unilamellar vesicles. The total change in heat capacity was 2 to 3% depending on the system.

nada - nadase反应的研究得到了全面的扩展