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DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES

DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES
开发用于溶液和细胞生化研究的生物量热计
批准号：
3878934
负责人：
R L BERGER
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3878934
关键词：
NAD nucleosidase microcalorimetry phospholipids reagent /indicator thermodynamics

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中文摘要
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英文摘要
Studies of the reaction of NAD-NADASE have been extended on the all tantalum stopped-flow microcalorimeter to 10 degrees C, 25 degrees C, and 37 degrees C confirming the endothermic nature of the reaction by demonstrating the 5-fold increase in activity from 25 degrees C to 10 degrees C with the almost complete loss in activity at 37 degrees C. A new heat capacity measuring microcalorimeter has been developed which is capable of measuring charges of 1 part in one hundred thousand of total heat capacity of phospholipids. A reaction heat of 60 calories/mole was found as they went from multilamellar vesicles to unilamellar vesicles. The total change in heat capacity was 2 to 3% depending on the system.
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DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDYING BIOCHEMICAL REACTIONS
DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MOLECULAR REACTIONS
DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES
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