DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS

开发用于监测酶-底物反应的近红外光谱

• 批准号: 3858005
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858005
• 关键词: adenosine diphosphate; adenosine triphosphate; bioengineering /biomedical engineering; chemical reaction; enzyme mechanism; enzyme substrate complex; infrared spectrometry; phosphates

We have employed near infrared spectroscopy to quantify each of the compounds ATP,ADP, and PO 4. We have successfully made calibration curves for each in the 1 to 25 micromolar regime. Recently, mixtures of the three phosphate compounds were also quantified in the same concentration region. Hence, the distinction of ATP, ADP, and P(i) can be achieved with near infrared. Because there are significant variations in the near infrared spectra of ATP and ADP and the majority of change between ATP and ADP lies within the phosphate moiety, it follows that the differences being followed in the near infrared spectra of ADP and ATP lie within this environment.

我们已经采用了附近的红外光谱法来量化每个 化合物ATP，ADP和PO 4。我们已成功制作了校准曲线 对于1至25个微摩尔制度中的每个人。 最近，三个混合物 在同一浓度区域也定量了磷酸化合物。 因此，可以在接近的情况下实现ATP，ADP和P（i）的区别 红外线的。 因为近红外有很大的变化 ATP和ADP的光谱以及ATP和ADP之间的大部分变化位置 在磷酸盐部分中，遵循的差异 在ADP和ATP的近红外光谱中，在这种环境中。