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DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS

DEVELOPMENT OF NIR FOR MONITORING ENZYME-SUBSTRATE REACTIONS
开发用于监测酶-底物反应的近红外光谱
批准号：
3858005
负责人：
R L BERGER
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3858005
关键词：
adenosine diphosphate adenosine triphosphate bioengineering /biomedical engineering chemical reaction enzyme mechanism enzyme substrate complex infrared spectrometry phosphates

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英文摘要
We have employed near infrared spectroscopy to quantify each of the compounds ATP,ADP, and PO 4. We have successfully made calibration curves for each in the 1 to 25 micromolar regime. Recently, mixtures of the three phosphate compounds were also quantified in the same concentration region. Hence, the distinction of ATP, ADP, and P(i) can be achieved with near infrared. Because there are significant variations in the near infrared spectra of ATP and ADP and the majority of change between ATP and ADP lies within the phosphate moiety, it follows that the differences being followed in the near infrared spectra of ADP and ATP lie within this environment.
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