DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS

研究生物大分子反应的生物物理方法的发展

• 批准号: 3942821
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942821
• 关键词: acidity /alkalinity; adenosinetriphosphatase; cell membrane; chemical reaction; enzyme substrate; fiber optics; hemoglobin; lasers; muscle contraction; sickle cell anemia; stop flow technique; thermodynamics; viscosity

The adaptation of the isoionic hemoglobin preparation developed in this Laboratory by the joint Forces Casualty Care Blood Resources Division based at Letterman Army Research Institute has provided stimulus to a renewed interest in several instruments developed in this laboratory. A modified oxygen binding to hemoglobin apparatus has been set up and is presently undergoing tests in the Laboratory of Molecular Biology, NIA. A highly modified cell is nearing completion of which will permit the measurement of the oxygen dissociation curve of hemoglobin simultaneously by a manometric method and spectroscopy. Such data are needed if the various models of how hemoglobin binds oxygen are to be critically tested. In collaboration with computational scientists at UCLA and DCRT a considerable effort has been mounted to have all of the needed programs operating on the local IBM AT rather than a main frame computer. The inertial drive quench flow apparatus and thermal-optical stopped flow apparatus have had a number of improvements made to them over the last year and it is expected that the quench flow machine will go operational in the laboratory of Biological Chemistry, NIA by the end of the summer. It has become clear that for the thermal stopped flow to be useful a differential version will need to be built and this should be finished in the next year. Experiments are planned on the ATPase ATP reactions of muscle as a test of the system.

研制的异离子血红蛋白制剂的改进 在这个实验室由联合部队伤亡护理血液 莱特曼陆军研究所（Letterman Army Research Institute） 刺激了对若干文书的新兴趣 在这个实验室里开发的。 一种修饰的氧结合， 血红蛋白仪已经建立，目前正在进行 在分子生物学实验室，NIA。 一个高度 修改后的细胞即将完成，这将允许 血红蛋白氧解离曲线的测定 同时通过测压法和光谱法。 等 如果血红蛋白如何结合的各种模型 氧气将受到严格的测试。 协同 加州大学洛杉矶分校和DCRT的计算科学家 已经做出努力， 在本地IBM AT上运行，而不是在主机上运行 电脑 惯性驱动淬火流动装置和热光学 止流装置已经进行了许多改进 在过去的一年里，他们预计，淬火流 这台机器将在生物实验室运行 化学，NIA在夏天结束。 很明显 为了使热停止流动有用， 一个版本将需要建立，这应该在下一个完成 年 实验计划在ATP酶ATP反应的 肌肉作为系统的测试。