DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS

研究生物大分子反应的生物物理方法的发展

• 批准号: 3942821
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942821
• 关键词: acidity /alkalinity; adenosinetriphosphatase; cell membrane; chemical reaction; enzyme substrate; fiber optics; hemoglobin; lasers; muscle contraction; sickle cell anemia; stop flow technique; thermodynamics; viscosity

The adaptation of the isoionic hemoglobin preparation developed in this Laboratory by the joint Forces Casualty Care Blood Resources Division based at Letterman Army Research Institute has provided stimulus to a renewed interest in several instruments developed in this laboratory. A modified oxygen binding to hemoglobin apparatus has been set up and is presently undergoing tests in the Laboratory of Molecular Biology, NIA. A highly modified cell is nearing completion of which will permit the measurement of the oxygen dissociation curve of hemoglobin simultaneously by a manometric method and spectroscopy. Such data are needed if the various models of how hemoglobin binds oxygen are to be critically tested. In collaboration with computational scientists at UCLA and DCRT a considerable effort has been mounted to have all of the needed programs operating on the local IBM AT rather than a main frame computer. The inertial drive quench flow apparatus and thermal-optical stopped flow apparatus have had a number of improvements made to them over the last year and it is expected that the quench flow machine will go operational in the laboratory of Biological Chemistry, NIA by the end of the summer. It has become clear that for the thermal stopped flow to be useful a differential version will need to be built and this should be finished in the next year. Experiments are planned on the ATPase ATP reactions of muscle as a test of the system.

研制的等离子血红蛋白制剂的适应性 在这个实验室里由联合部队救治伤员血液 莱特曼陆军研究所资源部 刺激了人们对几种工具重新产生的兴趣 在这个实验室里研发的。修饰后的氧结合到 已经建立了血红蛋白仪器，目前正在进行 NIA分子生物学实验室的测试。一位高度 修改后的电池即将完成，这将允许 血红蛋白氧解离曲线的测定 同时通过测压方法和光谱分析。是这样的 如果血红蛋白如何结合的各种模型需要数据 氧气将接受严格的测试。与 加州大学洛杉矶分校和DCRT的计算科学家们 已经开始努力使所有需要的计划 在本地IBM AT而不是大型机上运行 电脑。 惯性驱动失超流动装置和热光系统 停流装置已作了许多改进 在过去的一年里，预计熄灭的水流 机器将在生物实验室投入使用 化学，NIA在夏末之前。事情已经很清楚了 为了让热停止流动变得有用， 版本将需要建立，这应该是在下一个完成 年。计划进行关于ATPase-ATP反应的实验 肌肉作为系统的测试。