DEVELOPMENT OF RAMAN SPECTROSCOPY FOR ENZYME-SUBSTRATE REACTIONS

酶-底物反应拉曼光谱的发展

• 批准号: 3858004
• 负责人: R L BERGER
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3858004
• 关键词: Raman spectrometry; adenosine diphosphate; adenosine triphosphate; bioengineering /biomedical engineering; chemical reaction; enzyme mechanism; enzyme substrate analog; enzyme substrate complex; infrared spectrometry; magnesium; time resolved data

Raman Spectra of ATP and ADP were obtained at Sandia National Laboratories and at the University of New Mexico. These provide baseline spectra as we explore the feasibility of obtaining spectra of ATP and various ATP complexes and analogs using time resolved Raman Spectroscopy. We have shown that Raman difference spectra provide the sensitivity required for small perturbations in two relatively dilute samples such as the ATP complex with Mg and in comparison to ADP. The differences were known from infra-red spectroscopy which is done at very high phosphate concentration where complexing with other ATP or ADP molecules sometimes occurs making interpretation difficult.

ATP和ADP的拉曼光谱在桑迪亚国家实验室获得