ESTIMATION OF HETEROZYGOSITY FOR SINGLE PROBE, MULTIL. DNA FINGER.

单探针、多探针杂合性的估计。

• 批准号: 3853575
• 负责人: J C STEPHENS
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853575
• 关键词: DNA; alleles; chemical fingerprinting; genetic mapping; heterozygote; human population genetics; nucleic acid probes

One of the most useful parameters that can be derived from population genetic data is the average heterozygosity (H), which has traditionally been employed to contrast levels of genetic variation among populations and to compare genetic variation among different loci. In spite of the increasing application of DNA fingerprinting to natural populations and to the genetic identification of humans, explicit methods for estimation of H from DNA fingerprinting data have not been developed. Contributing to this omission is the inability to determine relatively important genetic information for multilocus fingerprinting probes, such as the number of loci, the number of alleles, and the distribution of these alleles into specific loci. We have derived explicit calculations for the expected average heterozygosity and a maximum value for this estimate. These estimates are based upon the DNA restriction pattern matrices that are typical for fingerprinting studies of humans and natural populations. For several empirical datasets from our laboratory, these estimates are shown to be relatively close to each other. This observation, and consideration of the effect of missing alleles and alternate numbers of loci, suggest that the average heterozygosity can be accurately approximated using phenotypic DNA fingerprint patterns because this parameter is relatively insensitive to the lack of certain genetic information.

可以从总体中得出的最有用的参数之一 遗传数据是平均杂合性（H），传统上 被用来对比种群间的遗传变异水平 并比较不同位点间的遗传变异。尽管有 DNA指纹技术越来越多地应用于自然群体， 对人类的遗传鉴定，明确的估计方法， DNA指纹数据的H尚未开发。 贡献 这一疏忽是无法确定相对重要的 多位点指纹探针的遗传信息，例如 基因座的数量，等位基因的数量，以及这些基因的分布， 等位基因进入特定的基因座。 我们已经推导出了 期望的平均杂合性和该杂合性的最大值 估算 这些估计是基于DNA限制性图谱 矩阵是典型的指纹研究的人类和 自然人口。 对于我们实验室的几个经验数据集， 这些估计数显示彼此比较接近。 这 观察和考虑缺失等位基因的影响， 交替数量的基因座，表明平均杂合性可以 使用表型DNA指纹模式准确地近似，因为 该参数对缺乏某些遗传因素相对不敏感， 信息.