INVESTIGATIONS OF GENETIC DISEQUILIBRIA AS GENE MAPPING STRATEGIES

作为基因图谱策略的遗传不平衡研究

• 批准号: 3752729
• 负责人: J C STEPHENS
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752729
• 关键词: African American; Chinese; Native Americans; biochemical evolution; caucasian American; computer simulation; ethnic group; gene frequency; genetic mapping; genetic markers; genetic polymorphism; human data; human genetic material tag; human population genetics; linkage mapping; statistics /biometry

Our research has focussed on developing a gene mapping strategy that capitalizes on linkage disequilibrium in recently admixed populations. It is a reasonably well-known population genetic phenomenon that populations formed by recent admixture of genetically divergent populations will harbor greatly elevated levels of linkage disequilibrium. However, the disequilibrium will exist between unlinked genes as well as linked genes. The latter observation suggests that any strategy that makes use of the disequilibrium as an indication of linkage will need to be optimized to discriminate between these two possibilities. In the course of developing our strategy (named MALD--mapping by admixture linkage disequilibrium), we have used a variety of mathematical and computer simulation approaches to identify parameters having the greatest influence on detectability of linkage disequilibrium. In particular, we ascertained values of each parameter that would allow efficient gene mapping, in concert with two proposed strategies for declaring linkage. Furthermore, we have constructed a polymorphic admixture typing map (PATMAP) of 257 genetic markers. These markers were scored on population samples of Caucasians, African-Americans, Asians (Chinese), and Amerindians (Cheyenne). A substantial proportion of the markers had large gene frequency differences between populations, confirming their relevance to the MALD approach.

我们的研究重点是开发一种基因定位策略