HUMAN HERPESVIRUS-6 (HHV-6), EPSTEIN-BARR VIRUS (EBV) AND HIV STUDIES

人类疱疹病毒 6 (HHV-6)、爱泼斯坦-巴尔病毒 (EBV) 和 HIV 研究

• 批准号: 3853412
• 负责人: D ABLASHI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853412
• 关键词: AIDS; Burkitt's lymphoma; Epstein Barr virus; Hodgkin's disease; Sjogren's syndrome; antiviral antibody; biomarker; bone marrow transplantation; chronic fatigue syndrome; enzyme linked immunosorbent assay; human herpesvirus 6; human immunodeficiency virus 1; human tissue; in situ hybridization; interleukin 1; latent virus infection; monoclonal antibody; serotyping; systemic lupus erythematosus; tissue /cell culture; tumor necrosis factor alpha; virus DNA; virus antigen; virus cytopathogenic effect; virus protein; virus replication

The infection of HHV-6 occurs very early in childhood (under one year) and the virus remains for life in a latent state. Using the peripheral mononuclear cells in culture from HIV-1-positive AIDS. chronic fatigue syndrome (CFS), systemic lupus erythematosus (SLE) patients and bone marrow transplant recipients, a high frequency of virus activation (40%) compared to normal donors (12%) was detected indicating active virus replication. HHV-6 was found to induce IL-1beta and TNF-alpha in human peripheral blood mononuclear cells. In contradistinction. HHV-6 has no effect on IL-6 induction. Cytokine induction appeared to be mediated by early viral protein. Cytokine production by HHV-6 in cells suggests how HHV-6 may contribute to disease manifestation. HHV-6 antigen-positive cells were detected by in situ hybridization and by monoclonal antibody assays in Hodgkin's disease, (HD). AIDS. SLE and Sjogren's syndrome patients' cells. The virus DNA-positive cells expressed CD38+, CD4+, and to a lower degree CD8+ and CD21+ cell receptors. Using ELISA and HHV-6 early protein (P41/38). sera from African Burkitt's lymphoma (ABL). HD and American Burkitt's lymphoma (AmBL) showed high frequency of antibody to P41/38 (ABL, 96.5%; HD, 62.5%) compared to normals (12%), whereas ELISA to HHV-6 late antigen (gpll0) failed to detect these differences. These findings indicated active HHV-6 replication and suggested a possible role of this virus in the pathogenesis of these malignancies. Thus. this assay may be a good marker for active and chronic HHV-6 infections.

HHV-6感染发生在儿童早期（一岁以下）。