STUDIES ON EPSTEIN-BARR VIRUS AND HTLV-III

Epstein-Barr 病毒和 HTLV-III 的研究

• 批准号: 3963420
• 负责人: D ABLASHI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963420
• 关键词: Chinese; Epstein Barr virus; Herpesviridae disease; Herpesvirus saimiri; T lymphocyte; antibody formation; cancer risk; cellular immunity; chromosome deletion; circular DNA; fibroblasts; human middle age (35-64); human tissue; immunodeficiency; immunofluorescence technique; immunoglobulin A; leukocyte activation /transformation; longitudinal human study; nasopharyngeal neoplasms; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; oncogenic virus; prognosis; serotyping; tissue /cell culture; umbilical cord; viral carcinogenesis; virus antigen; virus genetics; virus related neoplasm /cancer; virus replication

Epstein-Barr virus (EBV) associated with B-cell lymphomas was recently isolated from the plasma of AIDS patients in our laboratory. In view of this development and recent reports of a 30-fold increase in B-cell lymphomas in AIDS patients, we attempted to investigate infection and replication of HTLV-III in B-cells. We found that only (but not all) EBV genome-positive cells could be infected by HTLV-III, whether or not they possessed T4 receptors. Two EBV-positive lines became chronically infected with HTLV-III. Three EBV genome-negative cell lines (lacking T4 receptors) could not be infected with HTLV-III until they were converted to EBV positivity. Even after infection, however, the latter remained negative for T4 receptors, suggesting that the mechanism of infection of B-cells by HTLV-III does not involve T4 antigens. Alternatively, EBV may induce a receptor that may be required for HTLV-III infection. Results suggest the interaction of EBV and HTLV-III in B-cell lymphomas associated with AIDS. The specific role of megakaryocytes is not fully known except that they are involved in platelet formation. Three human megakaryocyte cell lines were found to contain the EBV genome. One of these lines also possessed approximately 3% EBV virus capsid antigens (VCA). This was the first report of the ability of megakaryocytes to replicate EBV, suggesting that this cell type possesses EBV receptors. Like the EBV genome-positive B-cells, the megakaryocyte lines were infectible with HTLV-III, further supporting the role of EBV in HTLV-III infection and adding another cell type for HTLV-III replication.

EB病毒（EBV）与B细胞淋巴瘤有关，最近 是从我们实验室的艾滋病患者血浆中分离出来的。 鉴于 这一发展和最近的报告显示，B细胞增加了30倍， 在艾滋病患者的淋巴瘤，我们试图调查感染， HTLV-III在B细胞中的复制。 我们发现，只有（但不是所有）EB病毒 基因组阳性细胞可以被HTLV-III感染，无论它们是否 拥有T4受体 两条EB病毒阳性线被慢性感染 HTLV-III 三种EBV基因组阴性细胞系（缺乏T4受体） 不能感染HTLV-III，直到他们转化为EBV 积极性 然而，即使在感染后，后者仍然是阴性的 T4受体，这表明B细胞感染的机制， HTLV-III不涉及T4抗原。 或者，EBV可以诱导 可能是HTLV-III感染所需的受体。 结果表明该 艾滋病相关B细胞淋巴瘤中EBV和HTLV-III相互作用 巨核细胞的具体作用尚不完全清楚，除了它们是 参与血小板的形成。 三种人巨核细胞系， 发现含有EB病毒基因组。 其中一行还拥有 约3%的EBV病毒衣壳抗原（VCA）。 这是第一 巨核细胞复制EBV的能力的报告，这表明， 这种细胞类型具有EBV受体。 比如EB病毒基因组阳性 B细胞，巨核细胞系可被HTLV-III感染，进一步 支持EBV在HTLV-III感染中的作用， HTLV-III复制型。