HUMAN HERPESVIRUS-6, EPSTEIN-BARR VIRUS, SIMIAN IMMUNODEFICIENCY VIRUS AND HIV

人类疱疹病毒 6、EPSTEIN-BARR 病毒、猿猴免疫缺陷病毒和 HIV

• 批准号: 3838329
• 负责人: D ABLASHI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838329
• 关键词: AIDS; B lymphocyte; Epstein Barr virus; HIV infections; Primates; antiviral antibody; cell bank /registry; chronic fatigue syndrome; genetic polymorphism; human herpesvirus 6; human immunodeficiency virus 1; human tissue; immunopathology; latent virus infection; lymphoma; nervous system infection; serotyping; simian immunodeficiency virus; tissue /cell culture; virus antigen; virus classification; virus protein; virus replication

The infection of HHV-6 occurs very early in childhood (under one year) and virus remains for life in a latent state. Using the peripheral mononuclear cells in culture from HIV-1-positive AIDS, chronic fatigue syndrome (CFS), systemic lupus erythematosus (SLE) patients and bone marrow transplant recipients, a high frequency of virus activation (40%) compared to normal donors (12%) was detected indicating active virus replication. HHV-6 was found to induce IL-1b and TNF-a in human peripheral blood mononuclear cells. In contradistinction, HHV-6 has no effect on IL-6 induction. Cytokine induction appeared to be mediated by early viral protein. Cytokine production by HHV-6 in cells suggests how HHV-6 may contribute to disease manifestation. HHV-6 antigen-positive cells were detected by in situ hybridization and by monoclonal antibody assays in Hodgkins disease, (HD), AIDS, SLE and Sjogren's syndrome patients' cells. The virus DNA-positive cells expressed CD38+, CD4+, and to lower degree CD8+ and CD21+ cell receptors. Using ELISA and HHV-6 early protein (P41/38), sera from African Burkitt's lymphoma (ABL), HD and American Burkitt's lymphoma (AmBL) showed high frequency of antibody to P41/38 (ABL, 96.5%; HD, 62.5%) compared to normals (12%), whereas ELISA to HHV-6 late antigen (gp110) failed to detect these differences. These findings indicated active HHV-6 replication and suggested a possible role of this virus in the pathogenesis of these malignancies. Thus, this assay may be a good marker for active and chronic HHV-6 infections.

HHV-6的感染发生在儿童早期（一岁以下） 病毒会终生潜伏。 使用外围 HIV-1阳性艾滋病患者的单核细胞培养，慢性疲劳 综合征（CFS），系统性红斑狼疮（SLE）患者和骨 骨髓移植受者，病毒激活频率高（40%） 与正常供体（12%）相比， 复制的 HHV-6能诱导人IL-1b和TNF-α的表达， 外周血单个核细胞 相比之下，HHV-6没有 对IL-6诱导的影响。 细胞因子诱导似乎是由 早期病毒蛋白 HHV-6在细胞中产生的细胞因子表明 HHV-6可能参与疾病表现。 HHV-6抗原阳性 用原位杂交和单克隆抗体检测细胞 霍奇金斯病（HD）、AIDS、SLE和Sjogren综合征的测定 病人的细胞。 病毒DNA阳性细胞表达CD 38+、CD 4+、CD 6+、CD 8+、CD 10+、CD 12+、CD 14+、CD 16+、CD 18+、CD 19 CD 8+和CD 21+细胞受体表达降低。用ELISA和HHV-6 早期蛋白（P41/38），非洲伯基特淋巴瘤（ABL）血清，HD 美国伯基特淋巴瘤（AmBL）抗体阳性率高 P41/38（ABL，96.5%; HD，62.5%）与正常（12%）相比，而 HHV-6晚期抗原（gp 110）的ELISA未能检测到这些差异。 这些发现表明HHV-6复制活跃，并提示 这种病毒在这些恶性肿瘤发病机制中的可能作用。 因此，该检测方法可能是活动性和慢性HHV-6的良好标志物 感染.