STUDIES ON EPSTEIN-BARR VIRUS AND HERPESVIRUS SAIMIRI

EB病毒和SAIMIRI疱疹病毒的研究

• 批准号: 4692313
• 负责人: D ABLASHI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692313
• 关键词: Chinese; Epstein Barr virus; Herpesviridae disease; Herpesvirus saimiri; T lymphocyte; antibody formation; cancer risk; cellular immunity; chromosome deletion; circular DNA; fibroblasts; human middle age (35-64); human tissue; immunodeficiency; immunofluorescence technique; immunoglobulin A; leukocyte activation /transformation; longitudinal human study; nasopharyngeal neoplasms; neoplasm /cancer genetics; neoplasm /cancer immunodiagnosis; neoplasm /cancer immunology; oncogenic virus; prognosis; serotyping; tissue /cell culture; umbilical cord; viral carcinogenesis; virus antigen; virus genetics; virus related neoplasm /cancer; virus replication

In studies of sera from 11 patients with acquired immunodeficiency disease (AIDS), transforming Epstein-Barr virus (EBV) was isolated from the plasma of four. The latter expressed HTLV-III antibody as well as significantly elevated levels of EBV antibodies. Uncultured peripheral mononuclear cells from these individuals contained 8-15% EBV-membrane antigen (MA)-positive cells. Transforming virus was isolated at various passage levels after culture. In comparison, healthy donors not at risk of developing AIDS did not contain MA-positive cells in the peripheral blood and no EBV could be isolated from plasma or cultured cells. None of the EBV-positive AIDS sera contained neutralizing antibody to EBV which were present in sera from healthy donors. This is the first report of the isolation of EBV from the plasma of AIDS patients. In response to a request from NASA, squirrel monkeys which were to be used on space lab flights 3 and 4 were tested for Herpesvirus saimiri (HVS) which is endemic in this species. NASA officials were concerned about the possibility of crew members being infected by HVS being shed by he monkeys within the close confines of the spacecraft. Sera of 150 individuals occupationally exposed to squirrel monkeys were tested for the presence of HVS, and 11 (7.3%) were found positive for antibodies to HVS late antigen (LA) in the immunofluorescence test. No other HVS antibodies were evident. Eight of the 11 sera also immunoprecipitated HVS proteins. These results suggest that replication of HVS either does not occur in humans, or is too minimal to elicit immune responses. Although no demographic patterns in the HVS-LA positives were noted, the majority (7/11) had worked at primate centers around the country for periods ranging from less than one year to 25 years. This was the first report of the detection of HVS antibodies in humans.

在对11例获得性免疫缺陷病患者血清的研究中， （AIDS），从血浆中分离转化性EB病毒（EBV）， 四口 后者表达HTLV-III抗体，以及显着 EB病毒抗体水平升高。 未培养的外周血单个核细胞 从这些个体中含有8-15%的EBV膜抗原（MA）阳性 细胞 转化病毒在不同传代水平下分离， 文化 相比之下，没有患艾滋病风险的健康献血者却没有。 外周血中不含MA阳性细胞， 分离自血浆或培养的细胞。 EB病毒阳性的艾滋病血清中 含有EB病毒的中和抗体，这些抗体存在于 健康的捐赠者 这是首次报道EB病毒的分离， 艾滋病患者的血浆。 为了响应美国宇航局的要求，松鼠猴将被用于 在太空实验室飞行3和4测试了疱疹病毒saimiri（HVS） 这是这个物种特有的 美国宇航局官员担心， 船员被猴子传染人类疱疹病毒的可能性 在太空船的狭小范围内。 150人的血清 职业接触松鼠猴的人进行了测试， HVS晚期抗原抗体阳性11例（7.3 (LA)在免疫荧光测试中。 未检测到其他HVS抗体。 很明显。 11份血清中的8份也免疫沉淀HVS蛋白。 这些 结果表明，HVS的复制要么不发生在人类中，要么 太小了不足以引发免疫反应 虽然没有人口统计学 HVS-LA阳性者的模式被注意到，大多数（7/11）已工作 在全国各地的灵长类动物中心， 一年到二十五年。 这是首次报道检测到HVS 人体内的抗体