STUDIES ON HBLV, EBV, AND HIV

HBLV、EBV 和 HIV 研究

• 批准号: 3916752
• 负责人: D ABLASHI
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916752
• 关键词: AIDS; AIDS /HIV test; B lymphocyte; Burkitt's lymphoma; CD4 molecule; Epstein Barr virus; Herpesviridae disease; RNA directed DNA polymerase; T lymphocyte; antiviral antibody; cancer risk; human herpesvirus 6; human immunodeficiency virus 1; human tissue; immunofluorescence technique; lymphoma; neoplasm /cancer genetics; neoplasm /cancer immunology; oncogenic virus; serotyping; tissue /cell culture; viral carcinogenesis; virus DNA; virus antigen; virus cytopathogenic effect; virus genetics; virus receptors; virus related neoplasm /cancer; virus replication; virus virus interaction

Human B-lymphotropic (human herpesvirus-6) virus (HBLV), isolated from acquired immunodeficiency syndrome (AIDS) patients and patients with other malignancies and lymphopro-liferative disorders, known to possess B-cell surface markers, was also found to infect T-cells obtained from peripheral blood, bone marrow, spleen, thymus and tonsils. HBLV could also replicate and produce large quantities of virus or viral antigens in established B-, T- and megakaryocyte cell lines. Coincident infection by human immunodeficiency virus (HIV)-1 and HBLV of T-cells possessing T4 receptors showed enhanced cytopathic effects and enhanced reverse transcriptase activity of HIV. Both viruses could be found in the same T4 cell, suggesting that interaction between HIV-1 and HBLV could enhance impairment of immure function by accelerated destruction of target cells. These findings were supported by higher HBLV antibody in HIV-1-positive asymptomatic individuals (AIDS-related complex (ARC), persistent generalized lymphopathy (PGL) and AIDS) and the presence of HIV-l DNA in about 80% of peripheral lymphocytes from AIDS patients. We also found that HBLV could oriy infect Epstein-Barr virus (EBV)-genome-positive B-cell lines or B-cell lines converted to ESV positivity, suggesting that EBV induces a receptor common for HIV and HBLV. HBLV and EBV antibody titers in African Burkitt's lymphoma (BL) sera were elevated: seven of ten BL tumors contained ESV and HBLV DHA. This suggests that HBLV has a role as a cofactor in some B-ce11 tumors. HBLV DNA was a1so detected in two Sjogren's B-cell tumors and a large B-cell follicular lymphoma. These findings are compatible with the implication of HBLV in some malignant, lymphoproliferative and immunosuppressive disorders.

分离的人B淋巴细胞(人疱疹病毒-6)病毒(HBLV) 来自获得性免疫缺陷综合征(艾滋病)患者和 患有其他恶性肿瘤和淋巴增殖症的患者 已知具有B细胞表面标志的疾病也被发现 感染从外周血、骨髓获取的T细胞， 脾、胸腺和扁桃体。HBLV也可以复制和生产 在已建立的B-，T-中存在大量病毒或病毒抗原 和巨核细胞系。人类偶合感染 携带T4的T细胞中的HIV-1和HBLV 受体表现出增强的细胞病变效应和增强的逆转作用 HIV转录酶活性。这两种病毒都可以在 同样的T4细胞，提示HIV-1和HBLV之间的相互作用 可通过加速增强免疫功能的损害 破坏目标细胞。这些发现得到了 HIV-1阳性无症状个体HBLV抗体升高 (艾滋病相关复合体(ARC)，持续性全身性淋巴病变 (前列腺癌和艾滋病)和约80%的人中存在艾滋病毒-L DNA 艾滋病患者的外周血淋巴细胞。我们还发现HBLV 奥利伊能感染EB病毒基因组阳性的B细胞吗 转化为ESV阳性的细胞系或B细胞系，表明 EBV诱导HIV和HBLV共同的受体。HBLV和EBV 非洲Burkitt淋巴瘤(BL)血清抗体效价为 升高：10个BL肿瘤中有7个含有ESV和HBLV DHA。这 提示HBLV在某些B-CE11肿瘤中起辅助因子的作用。 在两例干燥B细胞瘤和一例干燥B细胞瘤中也检测到HBLV DNA 大B细胞滤泡性淋巴瘤。这些发现是一致的 HBLV在某些恶性、淋巴增生性疾病中的意义 和免疫抑制障碍。