MULTISTATION COMPUTERIZED MOTION ANALYSIS SYSTEM

要求提供经费，用于多用户计算机化的行动分析系统。 该系统由四个部分组成，图像处理和图形分析 通过以太网连接到中央处理器和文件服务器的工作站， a太阳微系统公司SPARCstation 330。软件将是2-D ExpertVision 用于分析细胞和粒子运动的系统（EV），以及动态 用于分析细胞形状变化的形态学系统（DMS）（均来自 Motion Analysis Systems，Inc.）。Zeiss Axiovert 35显微镜 要求高分辨率的微分图像对比度（DIC），相位， 暗场和荧光图像。 主要应用将是分析（i）趋光性信号传导， 盐生盐杆菌和莱茵衣藻中的视紫红质，（ii） 利用光不稳定性研究大肠杆菌趋化性中的激发信号 笼化的化学效应物，（iii）趋化性和细胞形状的变化， 盘基网柄藻，（iv）细菌和鱿鱼中的膜马达 轴突，和（v）游泳行为的完整和透化的paramericans。 主要用户群包括Spudich博士、Condeelis博士、Khan博士、Satir博士， 还有西格尔所要求的硬件和软件将包括现有的 从我们的单个实验室的有限能力的组件到一个 近两个数量级的多站运动分析网 更快的计算速度。

Funds are requested for a multiuser computerized motion analysis system. The system consists of four image processing and graphics analysis workstations linked via ethernet to a central processor and file server, a Sun Microsystems SPARCstation330. Software will be the 2-D ExpertVision System (EV) for the analysis of cell and particle motion, and the Dynamic Morphology System (DMS) for the analysis of cell shape changes (both from Motion Analysis Systems, Inc.). One Zeiss Axiovert 35 microscope is also requested for highly resolved differential image contrast (DIC), phase, darkfield, and fluorescence images. The main applications will be to analyze (i) phototaxis signaling by rhodopsins in Halobacterium halobium and Chlamydomonas reinhardtii, (ii) excitation signaling in chemotaxis of Escherichia coli using photolabile caged chemoeffectors, (iii) chemotaxis and cell shape changes in Dictyostelium discoideum, (iv) membrane motors in bacteria and in squid axons, and (v) swimming behavior of intact and permeabilized paramecia. The major user group is comprised of Drs. Spudich, Condeelis, Khan, Satir, and Segall. The requested hardware and software will incorporate existing components of limited capability from our individual laboratories into a multistation motion analysis network with nearly two orders of magnitude greater computational speed.