METAL-PROTEINS STUDIES BY FLUORESCENCE SPECTROSCOPY AND HALIDE ION NMR

生化系统中金属离子的研究包括两个方面 酶反应的重要方面。首先， 金属酶和金属活化酶需要 金属的存在，以使酶进行其 功能；第二，酶系统被破坏或 被某些金属离子(主要是 重金属)。这项研究建议使用天然荧光 以收集关于这两种酶的信息 酶反应的各个方面。这意味着没有外部探测器 必须添加可能扰乱酶或扰乱酶的物质 金属的效果。这项研究使用的另一种方法是 卤素离子核磁共振。该技术可用于检测 并确定金属离子的可及性和局部 结合部位的运动。如果成功，这些技术 这项研究开发的药物将有助于设计治疗癌症的新药 治疗重金属中毒，并将提供 关于金属离子在以下方面的作用的基本信息 金属酶和金属激活的生化系统。

The study of metal ions in biochemical systems encompasses two significant aspects of enzyme reactions. First, the metalloenzymes and metal activated enzymes that require the presence of the metal in order for the enzyme to carry out its function and second, the enzyme systems which are disrupted or inhibited by the presence of certain metal ions (primarily the heavy metals). This study proposes to use the native fluorescence of the amino in enzymes to gather information about these two aspects of enzyme reactions. This means that no external probe will have to be added which may perturb the enzyme or disrupt the effect of the metal. The other method used in this study is halide ion NMR. This technique can be used to detect binding of the metal ion and to determine the accessibility to and local motion at the binding site. If successful, the techniques developed in this study will help to design new drugs for the treatment of heavy metal poisoning and would provide fundamental information about the role of the metal ion in metalloenzymes and metal activated biochemical systems.