The Development of Capillary Electrochromatography

毛细管电色谱的发展

• 批准号: 6357609
• 负责人: JOSEPH J PESEK
• 金额: $ 14.7万
• 依托单位: SAN JOSE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6357609
• 关键词: aluminum oxide; analytical chemistry; atomic force microscopy; bioengineering /biomedical engineering; capillary electrophoresis; chemical kinetics; chemical stability; chemical structure function; conformation; electrical property; high performance liquid chromatography; intermolecular interaction; international cooperation; ions; ligands; physical separation; protein folding; protein purification; scanning electron microscopy; silicon; solute; spectrometry; surface property; technology /technique development; thermodynamics; titanium

DESCRIPTION (provided by applicant): This project involves a broad-based investigation into the utility of capillary electrochromatography (CEC) for biological and pharmaceutical analysis. The studies proposed include many practical applications and the development of the technique from its still rudimentary state. However, the data gathered in many of the experiments can be used to gain a better theoretical understanding of CEC. Both the fundamental and practical knowledge gained will be used to determine what types of analyses and analytes are suited to CEC. These investigations will involve both the packed column and the open tubular approaches to CEC. Early studies have already shown that each approach has its own particular advantages and disadvantages. The studies will focus on the preparation and development of new column materials for the packed capillary method and surface modifications for the open tubular method. The potential advances that can be achieved by CEC have broad implications for advancement of practical methodology in the health-related sciences.

描述（申请人提供）：该项目涉及广泛的基础 毛细管电色谱（CEC）用于 生物和药物分析。这些研究包括许多 介绍了该技术的实际应用和发展 基本状态然而，在许多实验中收集的数据可以 用于获得对CEC的更好的理论理解。基波 获得的实践知识将用于确定哪些类型的分析 并且分析物适合于CEC。这些调查将涉及 填充柱和开管法进入CEC。早期研究 已经表明，每种方法都有自己的特殊优势， 缺点这些研究将侧重于编写和开发新的 用于填充毛细管法的柱材料和表面改性 开管法CEC可以实现的潜在进步 对促进实用方法学的发展具有广泛的影响， 与健康有关的科学。

项目成果

Electrochromatographic studies of etched capillaries modified with a cyano pentoxy biphenyl liquid crystal.

用氰基戊氧基联苯液晶改性的蚀刻毛细管的电色谱研究。

• 发表时间: 2004
• 期刊: Journal of chromatography. A
• 作者: Dawson,GBrent;Matyska,MariaT;Pesek,JosephJ;Seipert,RichardR
• 通讯作者: Seipert,RichardR

Hydride-based silica stationary phases for HPLC: fundamental properties and applications.

用于 HPLC 的氢化物二氧化硅固定相：基本特性和应用。

• DOI: 10.1002/jssc.200500201
• 发表时间: 2005
• 期刊: Journal of separation science
• 影响因子: 3.1
• 作者: Pesek,JosephJ;Matyska,MariaT
• 通讯作者: Matyska,MariaT

Open-tubular electrochromatographic characterization of synthetic peptides.

合成肽的开管电色谱表征。

• DOI: 10.1002/elps.200405893
• 发表时间: 2004
• 期刊: Electrophoresis
• 影响因子: 2.9
• 作者: Pesek,JosephJ;Matyska,MariaT;Dawson,GBrent;Chen,JennyI-Chen;Boysen,ReinhardI;Hearn,MiltonTW
• 通讯作者: Hearn,MiltonTW

Open tubular capillary electrochromatography migration behavior of enkephalins in etched chemically modified fused silica capillaries.

• DOI: 10.1016/j.chroma.2006.04.090
• 发表时间: 2006-09
• 期刊: Journal of chromatography. A
• 作者: J. Pesek;M. Matyska;S. Velpula

HPLC retention behavior on hydride-based stationary phases.

• DOI: 10.1002/jssc.200600414
• 发表时间: 2007-03
• 期刊: Journal of separation science
• 影响因子: 3.1
• 作者: J. Pesek;M. Matyska;S. Larrabee