DETERMINATION OF BINDING ENTHALPIES OF ANTHRACYCLINE DRUGS TO DNA HOST CELLS

蒽环类药物与 DNA 宿主细胞结合焓的测定

• 批准号: 3896190
• 负责人: C P MUDD
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3896190
• 关键词: DNA; calorimetry; chemical binding; daunorubicin; drug interactions; stop flow technique; tantalum; thermodynamics

After performing the first direct measurements of the drug binding enthalpies for daunomycin to DNA in the 10-20 micromolar range (where the monomeric form predominates), we extended the experiment to other anthracycline drugs. We have now studied the salt and temperature dependence of the binding enthalpies for this class of drug. With a tantalum stopped flow microcalorimeter, we were able to measure 120 of these runs per day with an uncertainty of only 3 microjoules.

在执行药物结合的第一次直接测量之后 柔红霉素对DNA的热焓在10-20微摩尔范围(其中 单体形式占主导地位)，我们将实验扩展到其他 蒽环类药物。我们现在已经研究了盐和温度 对这类药物的结合热的依赖性。使用一个 钽止流微量热计，我们能够测量到120 这些仪器每天运行，不确定度只有3微焦。