DETERMINATION OF BINDING ENTHALPIES OF ANTHRACYCLINE DRUGS TO DNA HOST CELLS

蒽环类药物与 DNA 宿主细胞结合焓的测定

• 批准号: 3937343
• 负责人: C P MUDD
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937343
• 关键词: DNA; anthracyclines; bioengineering /biomedical engineering; calorimetry; chemical binding; daunorubicin; drug interactions; heat; salts; stop flow technique; tantalum; thermodynamics

The first set of measurements of the drug binding enthalpies for daunomycin to DNA in the 10-20 micromolar range (where the monomeric form predominates) have been made. These results were presented in a poster session at the 1988 meeting of the Biophysical Society. The enthalpies at these concentrations were in the 30-50 microjoule range. With a tantalum stopped flow calorimeter, we were able to measure 120 of these reactions per day with an uncertainty of only 3 microjoules. We anticipate two more sets of experiments to explore the salt and temperature dependence of the enthalpies. The experiments are being performed in collaboration with the Department of Chemistry at Rutgers University.

第一组药物结合焓的测量