MICROCALORIMETER MEASUREMENTS OF DNA-PROTEIN INTERACTIONS

使用各种蛋白质来修饰人免疫系统应答是有益的。 对器官移植、艾滋病和化疗领域有着浓厚的兴趣。 在NCI，已经为此目的开发了几种新药。在 目前，这些药物仅以少量（约1 ml）。通过将这些药物与精心构建的DNA分子混合， 通过测量焓，可以确定药物-DNA键的相互作用。 NCI和NIDDK的调查人员咨询了BEIB和LTD， 钽制断流微量热计， 80微升样品中10微焦耳或更低的热量。第一 实验表明，我们可以测量5微焦耳范围内的热量， 标准误差小于0.5微焦耳。初步结果显示 焓非常低，这表明结合几乎是 完全是熵驱动的

The use of various proteins to modify the human immune system response is of intense interest in the areas of organ transplants, AIDS, and chemotherapy. At the NCI, several new drugs have been developed for this purpose.. At present, these drugs are available only in small amounts (approximately 1 ml). By mixing these drugs with carefully constructed molecules ' of DNA and measuring the enthalpy, the drug-DNA bond interactions can be determined. Investigators at the NCI and NIDDK consulted BEIB and LTD in regards to tantalum stopped flow microcalorimeter which has the capability of measuring heats of 10 microjoules or less in 80 microliter samples. The first experiments showed that we could measure heats in the range of 5 microjoules with a standard error of less than 0.5 microjoules. Preliminary results show that the enthalpy is very low which indicates that the binding is almost exclusively entropy driven.