CALORIMETRIC INVESTIGATION OF DNA/ANTHRACYCLINE DRUG INTERACTIONS

DNA/蒽环类药物相互作用的量热研究

• 批准号: 3767438
• 负责人: C P MUDD
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3767438
• 关键词: DNA; anthracyclines; calorimetry; chemical binding; drug interactions; ionic strengths; stop flow technique; temperature; thermodynamics

After performing the first direct measurements of the binding enthalpies of the anthracycline drug daunomycin to DNA in the 10 to 20 micromolar range (where the monomeric form predominates), we extended the experiment to study the interaction with other DNA hosts at varying salt concentrations and temperatures. With a differential stopped-flow microcalorimeter, we were able to measure 120 of these enthalpies per day, with an uncertainty of only 3 microjoules. The enthalpy heats ranged from -20 microjoules (endothermic) to +50 microjoules (exothermic). The experiments have now been expanded to measure and compare a complete set of binding profiles for a series of DNA host complexes.

在首次直接测量结合焓后 蒽环类药物道诺霉素与DNA的结合力在10至20微摩尔 范围（其中单体形式占主导地位），我们扩大了实验 研究在不同盐浓度下与其他DNA宿主的相互作用 浓度和温度。 有一个差动断流器 我们用微量热计测量了120个这样的样品， 一天，不确定性只有3微焦耳。 焓加热 范围从-20微焦耳（吸热）到+50微焦耳 （放热）。 实验现在已经扩展到测量和 比较一系列DNA宿主的一整套结合谱 配合物