EFFECTS OF GLYCOL ETHERS ON BONE MARROW PARAMETERS

乙二醇醚对骨髓参数的影响

• 批准号: 3898086
• 负责人: H L HONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3898086
• 关键词: blood cell count; bone marrow; colony stimulating factor; cytotoxicity; dosage; erythropoiesis; ethers; ethylene glycols; gender difference; histopathology; iron metabolism; laboratory mouse; radiotracer; testis; toxin metabolism

Ethylene glycol (EG) or ethylene glycol monomethyl ether (EGMME) was administered by gavage to both sexes of B6C3F1 mice for 4 consecutive days at total doses of 200,400, and 1000 mg/kg body weight. Bone marrow parameters were examined on days 1,5, and 14 after their final treatment. Exposure to EG produced hypocellularity and suppression of granulocyte- macrophage progenitor (CFU-C) colony formation in both sexes on days 1 and 5 postexposure. Values returned to normal by day 14 in the female mice but not in the males. Erythropoiesis, as measured by 59 Fe incorporation and quantitation of erythroid precursors in culture (CFU- E), revealed no effect in female mice and affected male mice at the high dose only. In contrast, EGMME exposure in female mice resulted in inhibition of erythropoiesis. There was also a pronounced effect on white blood cells with decreased peripheral counts, and decreases in the number of CFU-C's cultured from marrow cells. The effect of EGMME was also seen at the lower dose levels and was sustained through the 14-day evaluation period. In addition, EGMME caused a 20% decrease in testicular weight, which was shown microscopically to be a segmented degeneration of seminiferous tubules, an effect not found with EG. This study demonstrates that EGMME is more myelotoxic in mice than EG and that pancytopenia is more pronounced in males, while erythropoiesis is more affected in females. These results were published in J. Environ. Path. Toxicol. Oncol,.8 (7) p. 27-38, 1988.

乙二醇（EG）或乙二醇单甲醚（EGMME）是 通过管饲法向两种性别的B6 C3 F1小鼠连续4次给药 总剂量为200、400和1000 mg/kg体重。骨髓 在最后一次治疗后的第1、5和14天检查参数。 暴露于EG产生细胞减少和粒细胞抑制， 第1天两种性别的巨噬细胞祖细胞（CFU-C）集落形成 和5次给药后。雌性动物的数值在第14天恢复正常 但在雄性中没有。红细胞生成，通过59 Fe测量 培养物中红系前体的掺入和定量（CFU-C） E），显示对雌性小鼠没有影响，而在高剂量下影响雄性小鼠。 剂量而已。相比之下，雌性小鼠中的EGMME暴露导致 抑制红细胞生成。对白色也有明显的影响 外周血细胞计数减少， 从骨髓细胞中培养出的CFU-C。EGMME也有一定的作用 并持续至14天评价 期此外，EGMME导致睾丸重量减少20%， 在显微镜下显示为节段性变性， 生精小管，EG未发现的效果。本研究 表明EGMME在小鼠中比EG更具骨髓毒性， 全血细胞减少症在男性中更明显，而红细胞生成更多 影响女性。这些结果发表在J. Environ上。路径 毒理学. Oncol.8（7）第27-38页，1988年。