EFFECTS OF D-LIMONENE ON ALPHA 2U-GLOBULIN IN RAT KIDNEY

D-柠檬烯对大鼠肾脏α2U-球蛋白的影响

• 批准号: 3918654
• 负责人: H L HONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3918654
• 关键词: adenocarcinoma; alpha globulin; androgens; chemical carcinogen; dietary constituent; disease /disorder model; enzyme linked immunosorbent assay; gender difference; hyaline substance; hyperplasia; kidney neoplasms; laboratory mouse; laboratory rat; nutrition related neoplasm /cancer; renal toxin; renal tubule; vegetable oils

d-Limonene is a natural component of a variety of foods and beverages and is found in many fruits, vegetables, meats, spices and other food items. Recently NTP conducted chronic two-year studies of d-Limonene in rats and mice and found there was clear evidence of carcinogenic activity for male F344 rats only as shown by increased incidences of tubular cell nyperplasia, adenomas and adenocarcinomas of the kidney. The response observed in male rats may be linked to specific renal pertubation of alpha 2U-g1obulin, unique to the male rat kidney. This study was performed to evaluate the hyaline droplet formation and the presence of alpha 2U-globulin in the kidney of male and female F344 rats after 14 days of gavage dosing at doses of 0, 75, 150, 300, 600 or 1200 mg/kg d-Limonene in corn oil. The alpha 2U-globulin was determined by the enzyme linked immunosoroent assay (ELISA) in the kidney homogenates. Total protein were measured in the same aliquots for alpha 2U-g1obu1in by the Lowry method. We have confirmed that d- Limonene produced significant dose-related increase of alpha 2U- globulin only in male rats. These results suggest that d-Limonene associated nephrotoxicity in male rats may be related to altered catabolism of alpha 2U-globulin, a low molecular weight protein synthesized by the liver under androgenic control.

d-柠檬烯是多种食品的天然成分 饮料，存在于许多水果、蔬菜、肉类、香料中 和其他食品。 最近 NTP 进行了为期两年的长期 在大鼠和小鼠中对 d-柠檬烯进行的研究发现， 雄性 F344 大鼠致癌活性的证据仅如图所示 肾小管细胞增生、腺瘤和 肾腺癌。 在雄性大鼠中观察到的反应 可能与 α2U-球蛋白的特定肾扰动有关， 雄性大鼠肾脏所特有。 这项研究的目的是 评估透明液滴的形成和 α 的存在 14 小时后雄性和雌性 F344 大鼠肾脏中的 2U-球蛋白 以 0、75、150、300、600 或 1200 剂量强饲给药的天数 mg/kg 玉米油中的 d-柠檬烯。 测定α2U-球蛋白 通过肾脏酶联免疫吸附测定 (ELISA) 匀浆。 总蛋白以相同的等份进行测量 通过 Lowry 方法测定 alpha 2U-g1obu1in。 我们已经确认，d- 柠檬烯产生显着的剂量相关的α2U-增加 球蛋白仅存在于雄性大鼠中。 这些结果表明 d-柠檬烯 雄性大鼠的相关肾毒性可能与改变 α2U-球蛋白（一种低分子量蛋白质）的分解代谢 由肝脏在雄激素控制下合成。