CEREBRAL PROSTAGLANDIN & ADENOSINE RECEPTORS LINKED TO ADENYLATE CYCLASE

脑前列腺素

• 批准号: 3945218
• 负责人: M SPATZ
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3945218
• 关键词: adenosine; adenylate cyclase; blood brain barrier; brain circulation; cerebral cortex; cerebrovascular system; cyclic AMP; electron microscopy; fatty acid biosynthesis; fluorescence; glia; histochemistry /cytochemistry; microcirculation; microscopy; neuropharmacology; prostaglandin E; prostaglandin F; prostaglandins; radioimmunoassay; tissue /cell culture; vascular endothelium; vascular smooth muscle; vasoactive agent

The interaction between adenosine and PGE2 receptors linked to adenylate cyclase was investigated in cultured endothelial cells derived from dissociated cerebral microvessels. PGE2 stimulated adenylate cyclase in dose dependent manner (1nm-100 mu M) while adenosine increased the activity of adenylate cyclase only at high concentration of the drug (0.01- 0.1mM). Increasing concentration of adenosine with PGE2 (10- 6M) had a statistically significant effect on adenylate cyclase stimulation only at lower doses of adenosine (1-10nM). The same concentrations of adenosine (1-10nM) alone did not enhance significantly the formation of cAMP. Those results indicate that both vasoactive substances adenosine and PGE2 have an effect on receptors linked to adenylate cyclase.

腺苷与前列腺素E_2受体的相互作用 培养内皮细胞腺苷环化酶的研究 来源于分离的脑微血管。 前列腺素E_2以剂量依赖方式刺激腺苷环化酶 (1 nm-100 mU M)，腺苷则增加酶活性。 腺苷环化酶仅在药物的高浓度下(0.01- 0.1 mm)。增加腺苷浓度与前列腺素E_2(10- 6m)对腺苷环化酶有显著的影响 仅在较低剂量的腺苷(1-10 nm)下刺激。相同 单独的腺苷浓度(1-10 nm)不会增加 值得注意的是阵营的形成。 这些结果表明，这两种血管活性物质腺苷 和PGE2对与腺苷环化酶相关的受体有影响。