INVESTIGATION OF EXTRANEURONAL CATECHOL SYNTHESIZING ENZYMES IN THE CNS

中枢神经系统外儿茶酚合成酶的研究

• 批准号: 4696910
• 负责人: M SPATZ
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696910
• 关键词: catecholamines; central nervous system; cerebrovascular system; epinephrine; exoenzyme; high performance liquid chromatography; histochemistry /cytochemistry; hormone biosynthesis; hormone regulation /control mechanism; microcirculation; neurotransmitter metabolism; norepinephrine; tissue /cell culture; vascular endothelium

Our previous immunohistochemical and biochemical studies of cerebral microvessels and cerebrovascular endothelial cultures showed the presence of phenylethanolamine-N-methyltransferase (PNMT) activity in both tissues. Since these extraneuronal tissues contain a catecholamine synthesizing enzyme which is responsible for conversion of norepinephrine to epinephrine, we extended these studies to determine whether vascular PNMT is indeed capable of producing epinephrine from norepinephrine. For this purpose a direct assay of endothelial epinephrine formed from norepinephrine was determined by using high pressure liquid chromatography. These studies, which are still in progress, have shown that the cultured cerebrovascular endothelium (2nd-4th generation) derived from dissociated cerebral microvascular fractions (obtained from rats) are capable of converting norepinephrine to epinephrine.

我们之前对脑的免疫组织化学和生化研究 微血管和脑血管内皮培养显示存在 两种组织中苯乙醇胺-N-甲基转移酶（PNMT）活性的变化。 由于这些神经元外组织含有合成儿茶酚胺 负责将去甲肾上腺素转化为 肾上腺素，我们扩展了这些研究以确定血管 PNMT 是否 确实能够从去甲肾上腺素产生肾上腺素。 为了这 目的 直接测定由以下物质形成的内皮肾上腺素 去甲肾上腺素采用高压液体测定 色谱法。 这些仍在进行中的研究表明 培养的脑血管内皮（第2-4代）衍生 来自分离的脑微血管部分（从大鼠获得） 能够将去甲肾上腺素转化为肾上腺素。