THE ESTABLISHMENT OF CEREBROVASCULAR SMOOTH MUSCLE CULTURE

脑血管平滑肌培养的建立

• 批准号: 4696918
• 负责人: M SPATZ
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696918
• 关键词: blood brain barrier; cell components; tissue /cell culture; vascular endothelium permeability; vascular smooth muscle

This study was concerned with the development and establishment of pure cerebrovascular smooth muscle culture. The established cell line originates from dissociated cells of microvessels obtained from brains of rats by mechanical dispersion and filtration technique. The cultured cells display histochemical and ultrastructural features characteristic of smooth muscle cells. They are as follows: an ovoid nucleus with one to four nucleoli and a granular, slightly basophilic perinuclear cytoplasm arranged in bundles throughout the cytoplasm, particularly adjacent to the opposing cellular membranes of the cells. In view of these observations, the cultured cerebrovascular smooth muscle cells provide a new model system for studying their function, especially related to the function of cerebral blood flow, blood pressure and overall to the blood-brain barrier function.

本研究涉及纯粹的开发和建立 脑血管平滑肌培养。 已建立的细胞系 源自从大脑中获得的微血管的分离细胞 大鼠通过机械分散和过滤技术。 培养的细胞显示组织化学和超微结构特征 平滑肌细胞的特征。 它们如下： 卵形 细胞核有 1 至 4 个核仁，颗粒状，略嗜碱性 核周细胞质在整个细胞质中成束排列， 特别是邻近细胞相对的细胞膜。 鉴于这些观察结果，培养的脑血管平滑肌 细胞为研究其功能，特别是 与脑血流、血压及整体功能有关 对血脑屏障功能。