EWING'S SARCOMA--DIFFERENTIATION IN VITRO

尤文肉瘤的组织发生仍然是个谜，尽管有很多工作， 阐明它的起源。 我们假设尤文氏肉瘤，在其通常的 分化状态，缺乏已知童年的任何特定特征 肿瘤的 来自其他研究的某些证据，例如 尤文氏肉瘤中的染色体易位（11：22）， 周围神经上皮瘤，以及与面板相似的反应模式 单克隆抗体，提出了一个可能的共同组织发生， 这些不同的肿瘤 由于神经肿瘤一般是 已知对分化剂如二丁酰环AMP有反应， 神经生长因子和视黄酸的发展特点， 分化的神经组织，如神经突和增加的数量， 致密核心颗粒，我们已经处理了一系列尤文肉瘤肿瘤细胞 在各种条件下，单独和 彼此结合。 迄今为止，初步结果强烈表明，至少这些肿瘤 这些在体外成功生长的细胞具有内在的神经功能 分化响应于用这些试剂的治疗。 四比四 这样研究的线（以前报告缺乏任何自发性证据 的神经分化，即使在体外生长一年后）， 在培养中产生细长的突起。 超微结构检查 这些过程揭示了致密的核心颗粒。 免疫细胞化学 神经元特异性烯醇化酶（一种在神经组织中发现的抗原）的抗血清， 所有四条线在治疗前均呈阴性，但治疗后均呈阳性。 这些初步结果正在用其他技术证实，包括 儿茶酚胺荧光，神经递质酶谱，细胞外 基质合成研究和单克隆抗体反应性模式。

The histogenesis of Ewing's sarcoma remains enigmatic, despite much work to elucidate its origins. We have assumed that Ewing's sarcoma, in its usual state of differentiation, lacks any specific features of known childhood tumors. Certain lines of evidence from other studies, such as the presence of a reciprocal (11:22) chromosomal translocation in Ewing's sarcoma and peripheral neuroepithelioma, and similar patterns of reactivity with panels of monoclonal antibodies, have suggested a possible common histogenesis for these otherwise dissimilar tumors. Since neural tumors in general are known to respond to differentiating agents such as dibutyryl cyclic AMP, nerve growth factor, and retinoic acid by developing features of differentiated neural tissues such as neurites and increased numbers of dense core granules, we have treated a series of Ewing's sarcoma tumor cell lines in vitro with these agents under a variety of conditions, alone and in conjunction with one another. To date, the initial results strongly suggest that at least those tumors which are successfully grown in vitro are intrinsically capable of neural differentiation in response to treatment with these agents. Four of four lines so studied (and reported previously to lack any spontaneous evidence of neural differentiation, even after year of growth in vitro) responded by producing long, slender processes in culture. Ultrastructural examination of these processes revealed dense core granules. Immunocytochemistry with antisera to neuron-specific enolase, an antigen found in neural tissue, was negative prior to treatment but positive afterwards in all four lines. These initial results are being confirmed with other techniques, including catecholamine fluorescence, neurotransmitter enzyme profiles, extracellular matrix synthesis studies, and patterns of monoclonal antibody reactivity.