SMALL, ROUND CELL TUMOR MONOCLONAL ANTIBODY REACTIVITY

小圆形细胞肿瘤单克隆抗体反应性

• 批准号: 4691722
• 负责人: T J TRICHE
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691722
• 关键词: Ewing's tumor; antileukocyte isoantibody; cellular oncology; flow cytometry; hematopoietic tissue; histochemistry /cytochemistry; immunochemistry; lymphoma; monoclonal antibody; neoplasm /cancer immunodiagnosis; neoplasm /cancer therapy; neuroblastoma; pediatric neoplasm /cancer; rhabdomyosarcoma

Primitive childhood tumors (i.e., classically Ewing's sarcoma, neuroblastoma, lymphoma, and soft tissue sarcoma), are frequently morphologically indistinguishable. Ultrastructural and immunocytochemical techniques are useful but not infallible. Monoclonal antibodies (MoAbs) which recognize neural, lymphoid (HLA-related), and tissue-specific determinants might be useful in distinguishing these entities. We have studied more than 20 cells lines by flow cytofluorometry and more than 40 tumors by frozen section immunocytochemistry with a panel of 12 MoAbs and find reproducible patterns of reactivity which serve to reliably distinguish all neural tumors and hematopoietic malignancies. Ewing's sarcoma is similar to rhabdomyosarcoma, but shows some reactivity with certain neural MoAbs. Peripheral neuroepithelioma is a unique tumor with reactivity intermediate between sarcomas and neural tumors, not unlike Ewing's sarcoma. Thus, most of the tumors are readily recognized, even in the absence of any distinguishing morphologic characteristic. These results have important diagnostic and therapeutic implications, but further study of more tumors is required.

原始儿童肿瘤（即，典型的尤文氏肉瘤， 神经母细胞瘤、淋巴瘤和软组织肉瘤）， 形态上难以区分。 超微结构和免疫细胞化学 技术是有用的，但不是绝对可靠的。 单克隆抗体（MoAb） 识别神经、淋巴（HLA相关）和组织特异性 决定因素可能有助于区分这些实体。 我们有 用流式细胞荧光法研究了20多个细胞系， 用一组12种单克隆抗体通过冷冻切片免疫细胞化学检测肿瘤， 找到可重复的反应模式， 区分所有神经肿瘤和造血系统恶性肿瘤。 尤文氏 肉瘤与横纹肌肉瘤相似，但与 某些神经MoAb 周围神经上皮瘤是一种独特的肿瘤， 反应性介于肉瘤和神经肿瘤之间， 尤因氏肉瘤。 因此，大多数肿瘤很容易识别，即使在 没有任何明显的形态特征。 这些 结果具有重要的诊断和治疗意义，但进一步 需要更多的肿瘤研究。