MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

放线菌菌毛粘附素的研究仍在继续 粘菌是一种口腔细菌，在牙齿表面定居， 牙菌斑，并有助于启动牙龈炎和牙周炎 疾病 放线菌1型菌毛介导细菌吸附 唾液处理的羟基磷灰石，牙齿表面的体外模型。 在这种相互作用中识别的宿主受体似乎是酸性的。 富含脯氨酸的蛋白质，存在于唾液中， 唾液膜 65千道尔顿1型菌毛的结构基因 亚基定位于放线菌DNA的1.9内切酶片段上 使用重组DNA技术。 编码的蛋白质与几种 针对1型菌毛的单克隆抗体，并已使用 用其中之一制备的亲和柱。 兔抗 克隆的菌毛亚基也与放线菌1型菌毛反应。 类型 2菌毛占细胞相关凝集素活性检测的 放线菌与乳酸菌乳糖敏感性共聚集 血链球菌 这种类型的相互作用似乎涉及到 细菌间粘附和特定微生物的形成 牙菌斑内的菌群。 2型菌毛亚单位的基因 已定位于放线菌DNA的2.5内切酶片段上。 它 编码一个59千道尔顿的蛋白质，该蛋白质已被纯化并与 几种针对2型菌毛的特异性单克隆抗体。 克隆的1型和2型菌毛亚单位参与了 正在检查每种菌毛的受体结合。 这些研究 已经开始为理解这些机制提供结构基础 口腔微生物粘附的证据

Studies are continuing to define the fimbrial adhesins of Actinomyces viscosus, an oral bacterium that colonizes tooth surfaces, forms dental plaque and contributes to the initiation of gingivitis and periodontal diseases. The type 1 fimbriae of Actinomyces mediate bacterial adsorption to saliva treated hydroxyapatite, an in vitro model of the tooth surface. The host receptors recognized in this interaction appear to be the acidic proline rich proteins found in saliva and as constituents of the acquired salivary pellicle. The structural gene for a 65 kilodalton type 1 fimbrial subunit has been localized on a 1.9 kilobase fragment of Actinomyces DNA using recombinant DNA technology. The encoded protein reacts with several monoclonal antibodies against type 1 fimbriae and has been purified using an affinity column prepared with one of these. Rabbit antibody against the cloned fimbrial subunit also reacts with Actinomyces type 1 fimbriae. Type 2 fimbriae account for the cell-associated lectin activity detected by the lactose sensitive coaggregation of Actinomyces with strains of Streptococcus sanguis. Interactions of this type appear to be involved in interbacterial adherence and the formation of specific microbial communities within dental plaque. The gene for a type 2 fimbrial subunit has been localized on a 2.5 kilobase fragment of Actinomyces DNA. It encodes a 59 kilodalton protein which has been purified and reacts with several specific monoclonal antibodies directed against type 2 fimbriae. The involvement of the cloned type 1 and type 2 fimbrial subunits in receptor binding by each type of fimbriae is being examined. These studies have begun to provide a structural basis for understanding the mechanisms of oral microbial adherence.