MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

对放线菌菌毛粘附素的研究仍在继续 粘液菌是一种定植于牙齿表面的口腔细菌，形成牙齿 菌斑并导致牙周炎和牙周病变 疾病。放线菌1型菌毛介导细菌吸附 唾液处理后的羟基磷灰石，一种牙齿表面的体外模型。 在这种相互作用中识别的宿主受体似乎是酸性的 唾液中发现的富含脯氨酸的蛋白质，以及作为获得性 唾液膜。65kodalton 1型菌毛的结构基因 亚基被定位在放线菌DNA的1.9kb片段上 使用重组DNA技术。编码的蛋白质与几个 抗1型菌毛的单抗，并已用 用这些中的一种制备的亲和柱。兔抗兔抗体 克隆的菌毛亚基也能与1型放线菌菌毛发生反应。类型 2菌毛解释了细胞相关凝集素活性 放线菌对乳糖敏感的共聚作用 血链球菌。这种类型的相互作用似乎涉及到 细菌间黏附与特定微生物的形成 牙菌斑内的群落。2型菌毛亚基的基因 已经定位在放线菌DNA的2.5kb的片段上。它 编码一种59kodalton的蛋白质，该蛋白质已被纯化并与 几种针对2型菌毛的特异性单抗。 克隆的1型和2型菌毛亚基参与 目前正在检查每种菌毛与受体的结合情况。这些研究 已经开始为理解这些机制提供了结构性的基础 口腔微生物的粘附性。

Studies are continuing to define the fimbrial adhesins of Actinomyces viscosus, an oral bacterium that colonizes tooth surfaces, forms dental plaque and contributes to the initiation of gingivitis and periodontal diseases. The type 1 fimbriae of Actinomyces mediate bacterial adsorption to saliva treated hydroxyapatite, an in vitro model of the tooth surface. The host receptors recognized in this interaction appear to be the acidic proline rich proteins found in saliva and as constituents of the acquired salivary pellicle. The structural gene for a 65 kilodalton type 1 fimbrial subunit has been localized on a 1.9 kilobase fragment of Actinomyces DNA using recombinant DNA technology. The encoded protein reacts with several monoclonal antibodies against type 1 fimbriae and has been purified using an affinity column prepared with one of these. Rabbit antibody against the cloned fimbrial subunit also reacts with Actinomyces type 1 fimbriae. Type 2 fimbriae account for the cell-associated lectin activity detected by the lactose sensitive coaggregation of Actinomyces with strains of Streptococcus sanguis. Interactions of this type appear to be involved in interbacterial adherence and the formation of specific microbial communities within dental plaque. The gene for a type 2 fimbrial subunit has been localized on a 2.5 kilobase fragment of Actinomyces DNA. It encodes a 59 kilodalton protein which has been purified and reacts with several specific monoclonal antibodies directed against type 2 fimbriae. The involvement of the cloned type 1 and type 2 fimbrial subunits in receptor binding by each type of fimbriae is being examined. These studies have begun to provide a structural basis for understanding the mechanisms of oral microbial adherence.