MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

与特异性粘附相关的微生物抗原

• 批准号: 4692619
• 负责人: J O CISAR
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692619
• 关键词: Actinomyces; Escherichia coli; Streptococcus; Streptococcus mutans; Streptococcus salivarius; Streptococcus sanguis; antibody formation; antibody specificity; bacterial antigens; cell aggregation; dental caries; erythrocytes; exo alpha sialidase; gingiva; gingivitis; human tissue; humoral immunity; hybridomas; immune complex; immunochemistry; membrane activity; microorganism immunology; monoclonal antibody; oral bacteria; periodontium disorder; surface antigens; tooth root

Studies are continuing to define the fimbrial adhesins of Actinomyces viscosus, an oral bacterium that colonizes tooth surfaces, forms dental plaque and contributes to the initiation of gingivitis and periodontal diseases. The type 1 fimbriae of actinomyces appear to be a principal adhesin for tooth surfaces as shown by studies of bacterial adsorption to saliva-treated hydroxyapatite. Recombinant DNA technology has been used to clone a structural gene that encodes the type 1 fimbrial subunit of A. viscosus strain T14V. This protein has a molecular weight of 62,000 daltons and is reactive with monospecific as well as monoclonal antibodies directed against actinomyces type 1 fimbriae. The type 2 fimbriae of actinomyces are antigenically and functionally distinct from type 1. Type 2 fimbriae account for the lactose-sensitive coaggregation of actinomyces with strains of Streptococcus sanguis and thus would be expected to promote interbacterial adherence within dental plaque. The gene encoding the type 2 fimbrial subunit of strain T14V has been cloned and the protein expressed in Eschedrichia coli has been partially purified. In addition to the study of type 2 fimbriae, immunochemical studies are continuing to define streptococcal carbohydrates that function as receptors for the actinomyces lectin. The receptor-associated polysaccharide of S. sanguis 34 has been purified and found to have N-acetylgalactosamine as the immunodominant group. These findings have begun to provide a structural basis for understanding the mechanisms of oral microbial adherence.

放线菌菌毛粘附素的研究仍在继续 粘菌是一种口腔细菌，在牙齿表面定居， 牙菌斑，并有助于启动牙龈炎和牙周炎 疾病 放线菌的1型菌毛似乎是一个主要的 细菌对牙齿表面的吸附研究表明， 唾液处理的羟基磷灰石。 重组DNA技术已被用于 克隆编码A. 粘菌菌株T14 V。 该蛋白质的分子量为62，000 道尔顿，并与单特异性以及单克隆抗体反应 针对放线菌1型菌毛。 2型菌毛 放线菌在抗原性和功能上不同于1型。 类型 2菌毛解释了放线菌乳糖敏感性共聚集 与血链球菌菌株接触，因此有望促进 牙菌斑内细菌间粘附。 编码这种类型的基因 2菌毛亚基的克隆及表达 在大肠杆菌中的表达已经部分纯化。 除研究 2型菌毛，免疫化学研究正在继续确定 作为放线菌受体的链球菌糖类 凝集素。 S. sanguis 34已经 纯化并发现具有N-乙酰半乳糖胺作为免疫显性 组 这些发现已经开始为以下方面提供结构性基础： 了解口腔微生物粘附的机制。