MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

革兰氏阳性细菌，主要是链球菌和放线菌，启动 口腔微生物定植，随后的牙菌斑形成和 牙周炎。这些生物事件代表着至关重要的 龋齿和牙周病的发展步骤取决于 特定的粘接性能，这是许多口腔常见的 细菌。其中一种相互作用是细菌与细菌之间的粘连 获得性表膜，一种启动牙齿定植的相互作用 由绿色链球菌所致。此前广泛的研究表明，这一点 过程涉及唾液酸反应性凝集素活性，并且这 活性还介导细菌与各种宿主细胞的粘连，包括 红血球。戈登链球菌凝集素活性的研究 现在被认为与一种特定的细菌细胞表面抗原有关。这 将Challis株的抗原性与自发性的 缺乏血凝活性的突变体。交叉 免疫电泳法显示在黄连提取物中存在一种天然抗原 在非粘附性突变体的提取物中不存在的亲本菌株。这个 纯化的抗原由蛋白质和碳水化合物组成，具有 根据标记的细胞的外观观察菌毛的形态 针对纯化的抗原制备的单特异性抗体。 值得注意的是，这种抗体的Fab片段抑制了凝集素介导的 细菌血凝，从而结合唾液酸结合 凝集素活性具有特定的细菌细胞表面结构。 链球菌胞壁多糖也可能在 口腔微生物定植，因为这些分子被 凝集素对其他细菌的作用。结构、免疫化学和遗传学研究 这些链球菌多糖正在进行中，以更清楚地定义 免疫学和凝集素识别的决定因素。洞察力 关于各种口腔微生物粘附剂的分子基础 相互作用很可能为控制斑块提供新的方法。 相关疾病。

Gram positive bacteria, primarily streptococci and actinomyces, initiate oral microbial colonization, subsequent dental plaque formation and gingival inflammation. These biological events, which represent essential steps in the development of caries and periodontal diseases, depend on specific adhesive properties, that are common to a number of oral bacteria. One such interaction is the adhesion of bacteria to the acquired pellicle, an interaction that initiates the colonization of teeth by viridans streptococci. Extensive previous studies have shown that this process involves a sialic acid reactive lectin activity and that this activity also mediates bacterial adhesions to various host cells including erythrocytes. The lectin activity of Streptococcus gordonii Challis has now been associated with a specific bacterial cell surface antigen. This involved the antigenic comparison of strain Challis with a spontaneous mutant that specifically lacked hemagglutinating activity. Crossed immunoelectrophoresis revealed a native antigen present in extracts of the parent strain that was absent in extracts of the nonadherent mutant. The purified antigen was composed of both protein and carbohydrate and had a fimbrial morphology based on the appearance of cells labeled with monospecific antibody prepared against the purified antigen. Significantly, Fab fragments of this antibody inhibited lectin-mediated bacterial hemagglutination, thereby associating the sialic acid binding lectin activity with a specific bacterial cell surface structure. Streptococcal cell wall polysaccharides may also play an essential role in oral microbial colonization since these molecules are recognized by lectins on other bacteria. Structural, immunochemical and genetic studies of these streptococcal polysaccharides are underway to more clearly define the determinants of immunological and lectin recognition. Insight concerning the molecular basis of various oral microbial adhesive interactions may well suggest novel approaches for the control of plaque related diseases.