MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

与特异性粘附相关的微生物抗原

• 批准号: 3753492
• 负责人: J O CISAR
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3753492
• 关键词: Actinomyces; Streptococcus; Streptococcus mitis; Streptococcus sanguis; bacterial antigens; bacterial polysaccharides; bacterial proteins; cell adhesion; dental caries; dental plaque; ecology; gene expression; genetic strain; gram negative bacteria; hydroxyapatites; lectin; microorganism interaction; molecular pathology; oral bacteria; receptor binding; receptor expression; saliva; surface antigens

Structural studies showed that the Streptococcus gordonii 38 receptor polysaccharide was composed of a repeating heptasaccharide linked end to end by phosphodiester bonds. The heptasaccharide repeating units of strain 38 and S. mitis J22 differed only at their reducing ends with GalNAcbeta13Gal in the former and Gal- beta13GalNAc in the latter while a rhamnose branch near the nonreducing end of the strain 38 heptasaccharide distinguished this structure from the linear hexasaccharide of S. oralis 34. Certain strains of S. sanguis and S. gordonii that express GalNAc-sensitive lectins coaggregated with strains 38 and 34 which have GalNAc- beta(13)Gal-containing receptor polysacchides but not with S. mitis J22 which has a Gal-beta13GalNAc containing receptor polysaccharide. Presumably, the complimentarity of these lectins involves the side of GalNAc-beta13Gal that includes the acetamido group of GalNAc-beta. In contrast, actinomyces interact with both types of receptor polysaccharides which suggests lectin complimentarity for a side of GalNAc-beta13Gal that is shared with Gal-beta13GalNAc. The antigenic properties of these polysaccharides appear to depend on structural features distinct from those detected by lectin binding. For example, the presence of the common rhamnose branch accounts for the strong cross reactivity between the strain 38 and J22 polysaccharides. A structural understanding of these cross reactions provides a rational basis for the preparation of specific immunological reagents. Studies were also initiated to characterize the adhesive properties of viridans streptococci that colonize the human oral cavity shortly following birth and also following the eruption of teeth. Whereas isolates obtained during the first two months were relatively nonadherent, isolates obtained after tooth eruption exhibited an array of adhesive properties similar to those noted in studies of adult isolates. The results clearly implicate specific bacterial adhesive properties as important determinants of in vivo colonization.

结构研究表明，格氏链球菌38 受体多糖由一个重复的七糖组成 通过磷酸二酯键首尾相连。 七糖 菌株38和S. mitis J22仅在以下方面不同： 它们的还原末端与GalNAc β 13 Gal在前者和Gal- β 13 GalNAc在后者，而鼠李糖分支附近的 菌株38七糖的非还原性末端区别于此， 结构来自S.口腔34. 某些 链球菌菌株sanguis和S.表达GalNAc敏感gordonii 凝集素与具有GalNAc的菌株38和34共聚集， β（13）Gal受体多糖，但不与S. 具有含Gal-beta13 GalNAc受体的缓症J22 多糖 据推测，这些凝集素的互补性 涉及GalNAc-β 13 Gal的一侧，其包括乙酰氨基 GalNAc-β组。 相反，放线菌与两者都相互作用， 表明凝集素的受体多糖类型 GalNAc-β 13 Gal的一侧的互补性，其与 Gal-beta13GalNAc. 它们的抗原特性 多糖似乎取决于不同的结构特征 与凝集素结合检测到的差异。 例如，存在 普通鼠李糖分支中的一个分支是强杂交的原因 菌株38和J22多糖之间的反应性。 一 对这些交叉反应的结构理解提供了 特异性免疫制剂制备的合理依据 试剂 还开始了研究，以表征粘合剂 定植于人类口腔的草绿色链球菌的特性 出生后不久，也在爆发后， 牙齿.而在前两个月获得的分离株， 牙齿萌出后获得的相对不粘附的分离物 显示出一系列的粘合性能，类似于 成年人隔离研究。 结果明显暗示了 细菌粘附特性作为体内 殖民化