MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

与特异性粘附相关的微生物抗原

• 批准号: 3775623
• 负责人: J O CISAR
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3775623
• 关键词: Actinomyces; Streptococcus; Streptococcus mitis; Streptococcus sanguis; bacterial antigens; bacterial polysaccharides; bacterial proteins; cell adhesion; dental caries; dental plaque; ecology; gene expression; genetic strain; gram negative bacteria; hydroxyapatites; lectin; microorganism interaction; molecular pathology; oral bacteria; receptor binding; receptor expression; saliva; surface antigens

Structural studies showed that the Streptococcus gordonii 38 receptor polysaccharide was composed of a repeating heptasaccharide linked end to end by phosphodiester bonds. The heptasaccharide repeating units of strain 38 and S. mitis J22 differed only at their reducing ends with GalNAcbeta13Gal in the former and Gal- beta13GalNAc in the latter while a rhamnose branch near the nonreducing end of the strain 38 heptasaccharide distinguished this structure from the linear hexasaccharide of S. oralis 34. Certain strains of S. sanguis and S. gordonii that express GalNAc-sensitive lectins coaggregated with strains 38 and 34 which have GalNAc- beta(13)Gal-containing receptor polysacchides but not with S. mitis J22 which has a Gal-beta13GalNAc containing receptor polysaccharide. Presumably, the complimentarity of these lectins involves the side of GalNAc-beta13Gal that includes the acetamido group of GalNAc-beta. In contrast, actinomyces interact with both types of receptor polysaccharides which suggests lectin complimentarity for a side of GalNAc-beta13Gal that is shared with Gal-beta13GalNAc. The antigenic properties of these polysaccharides appear to depend on structural features distinct from those detected by lectin binding. For example, the presence of the common rhamnose branch accounts for the strong cross reactivity between the strain 38 and J22 polysaccharides. A structural understanding of these cross reactions provides a rational basis for the preparation of specific immunological reagents. Studies were also initiated to characterize the adhesive properties of viridans streptococci that colonize the human oral cavity shortly following birth and also following the eruption of teeth. Whereas isolates obtained during the first two months were relatively nonadherent, isolates obtained after tooth eruption exhibited an array of adhesive properties similar to those noted in studies of adult isolates. The results clearly implicate specific bacterial adhesive properties as important determinants of in vivo colonization.

结构研究表明，戈登链球菌38 受体多糖由一个重复的七糖组成 通过磷酸二酯键连接在一起。七糖 菌株38和S.mitis J22的重复单位只有 他们的还原末端是前者的GalNAcbeta13Gal和Gal- β13GalNAc在后者中，而鼠李糖在靠近 菌株38的非还原末端七糖区分了这一点 口腔链霉菌34的线性六糖的结构。一定的 表达GalNAc敏感的血链球菌和戈登链球菌 凝集素与具有GalNAc-C的菌株38和34共同聚集 β(13)含半乳糖受体多糖，但不含S。 含有Gal-beta13GalNAc受体的Mitis J22 多糖类。想必，这些凝集素的赞誉 涉及GalNAc-Beta13Gal的一侧，其中包括乙酰胺 GalNAc-β组。相反，放线菌与两者相互作用。 提示凝集素的受体多糖的类型 对与之共享的GalNAc-Beta13 Gal的一面的赞扬 Galbeta13GalNAc。这些病毒的抗原性 多糖似乎依赖于不同的结构特征 从凝集素结合检测到的那些。例如，存在 的普通鼠李糖枝占强杂交 菌株38与J22多糖的反应性。一个 对这些交叉反应的结构理解提供了一个 制备特异性免疫学制剂的合理依据 试剂。 研究还开始了对粘合剂的表征 定植于口腔的绿色链球菌的特性 在出生后不久，也在喷发之后不久 牙。鉴于在前两个月获得的分离株是 牙萌出后获得的相对无粘附性的分离株 展示了一系列粘合剂特性，与 对成虫分离株的研究。这一结果清楚地暗示了具体的 细菌黏附特性是体内细菌黏附的重要决定因素 殖民主义。