DIFFERENTIATED AIRWAY EPITHELIAL CULTURES

单层非分化气管支气管上皮细胞培养物 有助于加深对疾病的理解， 囊性纤维化和哮喘，以呼吸道病毒感染为特征 上皮细胞，并推进我们对气管和支气管的认识 炎症此外，这种文化还使人们能够和/或促进 吸入毒理学研究，并允许深入了解肿瘤 气道的进展。分化的、分层的文化， 采用体内样表型预期将进一步增加这些 预付款。尽管如此，一个差异化的，商业上可用的模型， 气道上皮细胞不存在。 第一阶段的研究将调查生产组织的可行性 模拟其体内祖细胞的气道上皮细胞的培养模型 在结构和功能上。正常人源性气管支气管 上皮细胞将在单层培养中扩增，冷冻保存， 然后用于产生分层的、分化的组织培养物。 实验将调查各种文化参数和文化 将使用显微镜和免疫特异性技术进行表征。 最后，该模型的技术和商业可行性将是 评估。 拟议的商业应用：分化的组织培养模型 这里提出的将有助于筛选和测试新的 治疗各种疾病的药物和疗法， 影响气道上皮的疾病。此外，一种器官型 组织模型在毒理学、癌症和药物递送中是有用 问题研究

Monolayer, non-differentiated tracheo-bronchial epithelial cell cultures have been helpful in furthering the understanding of diseases such as cystic fibrosis and asthma, characterizing viral infection in the airway epithelia, and advancing our knowledge of tracheal and bronchial inflammation. In addition, such cultures have enabled and/or facilitated inhalation toxicology studies and allowed insight into neoplastic progression in the airways. Differentiated, stratified cultures which adopt an in vivo-like phenotype are anticipated to add further to these advances. Nonetheless, a differentiated, commercially available model of the airway epithelia does not exist. Phase I research will investigate the feasibility of producing a tissue culture model of the airway epithelia which mimics its in vivo progenitors in structure and function. Normal, human derived, tracheobronchial epithelial cells will be expanded in monolayer culture, cryopreserved, and then used to produce stratified, differentiated tissue cultures. Experiments will investigate a variety of culture parameters and cultures will be characterized using microscopic and immunospecific techniques. Finally, the technical and commercial viability of the model will be assessed. PROPOSED COMMERCIAL APPLICATIONS: The differentiated, tissue culture model here proposed would facilitate the screening and testing of new pharmacologic agents and therapies to combat the various diseases and disorders affecting the airway epithelia. In addition, an organotypic tissue model would be useful for toxicology, cancer, and drug deliver studies.