DIFFERENTIATED AIRWAY EPITHELIAL CULTURES

单层、未分化的气管-支气管上皮细胞培养 有助于加深对疾病的了解，如 囊性纤维化和哮喘：呼吸道病毒感染的特征 上皮细胞和促进我们对气管和支气管的认识 发炎。此外，这样的文化使和/或促进了 吸入毒理学研究和对肿瘤的洞察 呼吸道的进展。差异化、层次化的文化 采用体内类似的表型预计将进一步增加这些 预付款。尽管如此，一种差异化的、商业上可用的模型 呼吸道上皮细胞不存在。 第一阶段的研究将调查生产组织的可行性 模拟其体内祖细胞的呼吸道上皮细胞培养模型 在结构和功能上。正常，人源性，气管、支气管壁 上皮细胞将在单层培养中扩增，冷冻保存，并 然后用来生产分层的、分化的组织培养。 实验将调查各种培养参数和培养 将使用显微镜和免疫专一性技术进行表征。 最后，该模型的技术和商业可行性将是 评估过了。 建议的商业应用：差异化的组织培养模型 在此提出的建议将有助于筛选和测试新的 抗击各种疾病的药理制剂和疗法 影响呼吸道上皮细胞的疾病。此外，一种器官类型 组织模型将在毒理学、癌症和药物传递方面发挥作用 学习。

Monolayer, non-differentiated tracheo-bronchial epithelial cell cultures have been helpful in furthering the understanding of diseases such as cystic fibrosis and asthma, characterizing viral infection in the airway epithelia, and advancing our knowledge of tracheal and bronchial inflammation. In addition, such cultures have enabled and/or facilitated inhalation toxicology studies and allowed insight into neoplastic progression in the airways. Differentiated, stratified cultures which adopt an in vivo-like phenotype are anticipated to add further to these advances. Nonetheless, a differentiated, commercially available model of the airway epithelia does not exist. Phase I research will investigate the feasibility of producing a tissue culture model of the airway epithelia which mimics its in vivo progenitors in structure and function. Normal, human derived, tracheobronchial epithelial cells will be expanded in monolayer culture, cryopreserved, and then used to produce stratified, differentiated tissue cultures. Experiments will investigate a variety of culture parameters and cultures will be characterized using microscopic and immunospecific techniques. Finally, the technical and commercial viability of the model will be assessed. PROPOSED COMMERCIAL APPLICATIONS: The differentiated, tissue culture model here proposed would facilitate the screening and testing of new pharmacologic agents and therapies to combat the various diseases and disorders affecting the airway epithelia. In addition, an organotypic tissue model would be useful for toxicology, cancer, and drug deliver studies.