RECONSTRUCTED HUMAN GINGIVAL TISSUE
重建人类牙龈组织
基本信息
- 批准号:6014566
- 负责人:
- 金额:$ 10万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-07-01 至 2000-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Monolayer, non-differentiated gingival epithelial cell cultures have been helpful in furthering the understanding of various periodontal diseases such as such as gingivitis, oral cancer, and oral mucosal pathologies caused by smokeless tobacco. In addition, such cultures have enabled and/or facilitated biocompatibility studies for new dental implant materials and have led to potential therapies for the reepitheliaization of oral wounds. Differentiated, stratified cultures that adopt an in vivo-like phenotype are anticipated to add further to these advances. Nonetheless, differentiated, commercially available models of the gingival epithelia or oral mucosa do not exist. Phase I research will investigate the feasibility of producing a tissue culture model of the gingival epithelium which mimics its in vivo progenitor in structure and function. Normal, human derived, gingival epithelial and fibroblast cells will be harvested from primary tissue, expanded in monolayer culture, cryopreserved, and then used to produce stratified, differentiated organotypic cultures. Experiments will investigate a variety of culture parameters and the resultant cultures will be characterized using microscopic and immuno-specific techniques. Finally, the technical and commercial viability of the model will be assessed. PROPOSED COMMERCIAL APPLICATION: The differentiated tissue culture model here proposed would facilitate the screening and testing of new pharmacological agents and therapies to combat the various diseases and disorders affecting the gingiva. In addition, an organotypic tissue model of the gingival epithelium would be useful for dental implant biocompatibility, toxicology, and oral irritation studies.
单层非分化牙龈上皮细胞培养有助于进一步了解各种牙周疾病,如牙龈炎,口腔癌和由无烟烟草引起的口腔粘膜病变。此外,这样的培养物使得能够和/或促进新的牙科植入物材料的生物相容性研究,并导致口腔伤口上皮再生的潜在疗法。采用体内样表型的分化、分层培养有望进一步促进这些进展。尽管如此,牙龈上皮或口腔粘膜的分化的、可商购的模型并不存在。第一阶段的研究将探讨生产牙龈上皮组织培养模型的可行性,该模型在结构和功能上模仿其体内祖细胞。将从原代组织中收获正常的人源性牙龈上皮细胞和成纤维细胞,在单层培养物中扩增,冷冻保存,然后用于产生分层的分化器官型培养物。实验将研究各种培养参数,并使用显微镜和免疫特异性技术对所得培养物进行表征。最后,将评估该模型的技术和商业可行性。拟定商业应用:这里提出的分化组织培养模型将有助于筛选和测试新的药物制剂和治疗方法,以对抗影响牙龈的各种疾病和病症。此外,牙龈上皮的器官型组织模型将用于牙科种植体生物相容性、毒理学和口腔刺激研究。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MITCHELL KLAUSNER其他文献
MITCHELL KLAUSNER的其他文献
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Human Tissue Model For Heterosexual HIV Transmission
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